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HtrA Serine Peptidase-1 (HtrA1) ELISA, human

HtrA Serine Peptidase-1 (HtrA1) ELISA, human
Catalog Number: 
M046043
Qty/Size: 
96 wells

human TNF-alpha (TNFa) ELISA from MD Bioproducts

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For the quantitative determination of human HtrA1 in serum, tissue (Placenta) and cell culture supernatants.

Overview

Overview: 

Human HtrA1 protease was initially identified in human fibroblasts and belongs to the high temperature requirement factor A (HtrA) family of serine proteases that can be distinguished from other serine proteases by sequence homology, by the presence of a trypsin-type protease domain and one or two carboxyterminal PDZ domains and by their oligomeric architecture.

 

The loss of mammalian HtrA activity is correlated with severe diseases, including arthritis, cancer, familial ischemic cerebral small vessel disease and age related macular degeneration, as well as Parkinson’s disease and Alzheimer’s disease.

 

Epigenetic silencing occurs in various cancers, and the loss of HtrA1 correlates with decreased sensitivity to anticancer drugs and increased cell migration. In addition, overexpression of HtrA1 inhibited proliferation in vitro and tumor growth in vivo. These data suggest that HtrA1 might function as a tumor suppressor.

 

In the extracellular matrix, HtrA1 cleaves numerous secreted proteins, such as fibronectin, decorin, fibro- modulin, aggrecan, type II collagen, biglycan, clusterin, a disintegrin and metalloproteinase domain-contain- ing 9 (ADAM9), vitronectin, α-2-macroglobulin and the amyloid precursor protein fragment Aβ. The degra- dation of extracellular matrix components and the strong upregulation of HtrA1 in samples from patients implicate HtrA1 in arthritic diseases, in which it might affect the degradation of cartilage as well as inflam- mation.

 

It is unknown, how the cellular distribution of HtrA1 is regulated. In addition, little is known about the inter- action partners of mammalian HtrAs. It will be important to identify proteins that function as determinants of the cellular localization, substrate specificity and regulation of HtrA proteases.

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  serum, tissue, cell culture supernates

Sample Size: 500 uL

Standard Curve Range:  0.391 - 25 ng/mL

Sensitivity:  391 pg/ml

Assay Length:   4 hours

Literature/Support

Literature/Support: 

How It Works

How It Works: 

This quantitative assay is based on a two site sandwich format. A highly specific monoclonal antibody against HtrA1 is immobilised on the plate. HtrA1 will be bound to the wells, other components of the sample are removed by discarding/drying by taping and washing of the plate. The analyte is detected in two steps using a secondary biotin-labeled monoclonal antibody and a highly polymerised streptavidin-peroxidase conjugate. Any excess is removed by discarding/drying by taping and washing after each detection step. The amount of peroxidase bound to each well is determined by the addition of TMB Substrate. The reaction is stopped by adding the Stop Solution and the resultant color is read in a microplate reader at 450 nm. The concentration of HtrA1 in a sample is determined by interpolation from the standard curve.

 

human TNFa ELISA