web monitor

IL-6 ELISA, human

human IL-6 ELISA
Catalog Number: 
M046044
Qty/Size: 
96 wells

human TNF-alpha (TNFa) ELISA from MD Bioproducts

North America: 1-888-USMDBIO
International: +41-44 986 2628

Or you can order by using our online form
Have questions or need bulk? No problem, just ask.

For the quantitative determination of IL-6 in human plasma, serum and cell culture supernate samples.

Overview

Overview: 

Interleukin-6 (IL-6) is a cytokine of approximately 26 kDa that is synthesized by T-cells, macrophages, B-cells, fibroblasts, endothelial cells, and epithelial cells. IL-6 acts in both pro-inflammatory and anti-inflammatory ways. When released systemically it stimulates the liver to produce proteins, such as C-reactive protein and fibrin, which are responsible for the acute-phase response. Besides the systemic acute phase reaction, IL-6 is associated with several acute and chronic inflammatory diseases, including rheumatoid arthritis, acute pan- creatitis, viral and bacterial meningitis, and Alzheimer’s disease. However, IL-6 can also down regulate the inflammatory reaction by suppressing the pro-inflammatory cytokines IL-1 and TNF, and protect against lung damage and septic shock.

 

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  serum, plasma, cell culture supernates

Sample Size: 50 uL

Standard Curve Range:  0.0078 - 0.5 ng/mL

Sensitivity:  0.008 ng/mL

Assay Length:   4.5 hours

Literature/Support

Literature/Support: 

Product Insert:

human IL-6 ELISA Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

 

 

 

How It Works

How It Works: 

Human TNF-α ELISA kit is designed for detection of TNF-α in human plasma, serum or cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures TNF-α in less than 5 hours. A murine monoclonal antibody specific for human TNF-α has been pre-coated onto a microplate. TNF-α in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human TNF-α, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

 

human TNFa ELISA