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IL-8 ELISA, human

human IL-8 ELISA
Catalog Number: 
M046048
Qty/Size: 
96 wells

human TNF-alpha (TNFa) ELISA from MD Bioproducts

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For the quantitative determination of IL-8 in human plasma, serum tissue extracts and cell culture supernate samples.

Overview

Overview: 

CXCL8 or Interleukin-8 (IL-8) is a member of the CXC chemokine subfamily of cytokines. This basic heparin- binding protein precursor contains 99 amino acids and the mature functional protein comprises 72 amino acids. IL-8 is proinflammatory and primarily mediates the activation and migration of neutrophils from pe- ripheral blood into the sites of inflammation, injury, or infection in the tissue. IL-8 interacts with two receptors, CXCR1 and CXCR2, to activate leukocytes. Upon activation, both receptors couple to G protein to mediate phosphoinositide-hydrolysis, intracellular Ca2+ mobilization, chemotaxis, and exocytosis. CXCR1 is specific for IL-8 and activates phospholipase D and mediates respiratory burst. IL-8 is involved in a wide variety of physiological and pathological processes, including host defense against bacterial infection, bronchiolitis, ar- teriosclerosis, autoimmune disorders of skin, bones, and joints, and angiogenesis-dependent disorders such as rheumatoid arthritis, tumor growth, and wound repair.

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  serum, plasma, cell culture supernates

Sample Size: 50 uL

Standard Curve Range:  0.016 - 0.5 ng/mL

Sensitivity:  0.015 ng/mL

Assay Length:   < 5 hours

Literature/Support

Literature/Support: 

Product Insert:

human IL-8 Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

 

 

 

How It Works

How It Works: 

Human TNF-α ELISA kit is designed for detection of TNF-α in human plasma, serum or cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures TNF-α in less than 5 hours. A murine monoclonal antibody specific for human TNF-α has been pre-coated onto a microplate. TNF-α in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human TNF-α, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

 

human TNFa ELISA