web monitor

Prolactin (rat) ELISA

rat prolactin ELISA
Catalog Number: 
M046017
Qty/Size: 
96 wells

rat prolactin ELISA from MD Bioproducts

North America: 1-888-USMDBIO
International: +41-44 986 2628

Or you can order by using our online form
Have questions or need bulk? No problem, just ask.

The Prolactin (PRL) ELISA kit is designed to measure Rat Prolactin levels in serum and plasma.

Overview

Overview: 

Prolactin (PRL) or luteotropic hormone (LTH) is a peptide hormone with a molecular weight of 24,000 daltons and a structure that is similar to that of growth hormone and placental lactogen. It is primarily associated with pregnancy and lactation and is expressed in the anterior pituitary, uterus and placenta. During pregnancy, high circulating concentrations of estrogen promote prolactin production. The resulting high levels of prolactin secretion cause further maturation of the mammary glands, preparing them for lactation. 

Data/Specifications

Data/Specifications: 

Species: rat

Sample Type:   serum, plasma

Sample Size: 50 uL

Standard Curve Range:  0.39 - 50 ng/mL

Sensitivity:  0.2 ng/mL

Assay Length:   overnight incubation

Literature/Support

Literature/Support: 

Product Insert:

Rat Prolactin ELISA Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

References/Citations:How the Rat Prolactin ELISA Kit was used:
Temozolomide-induced inhibition of pituitary adenoma cells. Sheehan J, et al. Journ. of Neurosurgery. 2010Measure the concentration of prolactin in rat pituitary adenoma cell cultures (cell line GH3 and MMQ from the American Type Culture Collection).
Time-dependent alterations of peripheral immune parameters after nigrostriatal dopamine depletion in a rat model of Parkinson's disease. Engler H, et al. Brain, Behavior, and immunity. 2009;23(4): 518-26.Measure the concentration of prolactin in the plasma of adult male Fischer 344 rats.
Dopamine partial agonist actions of the glutamate receptor agonists LY 354,740 and LY 379,268. Seeman P, et al. Synapse. 2008; 62(2): 154-8.
Measure the concentration of prolactin in isolated anterior pituitary cells in primary tissue culture. Pituitary cells were isolated from Sprague-Dawley rats.
Memantine agonist action at dopamine D2High receptors. Seeman P, et al. Synapse. 2008; 62(2): 149-53.
Measure the concentration of prolactin in isolated anterior pituitary cells in primary tissue culture. Pituitary cells were isolated from Sprague-Dawley rats.

How It Works

How It Works: 

This Enzyme Immunoassay (EIA) is based on the competition between unlabelled rat prolactin and acetylcho- linesterase (AChE) linked to rat prolactin (tracer) for limited specific rabbit anti-rat prolactin antiserum sites. The complex rabbit antiserum-rat prolactin (free prolactin or tracer) binds to the mouse monoclonal anti-rabbit antibody that is attached to the well. The plate is then washed and Ellman’s Reagent (enzymatic substrate for AChE and chromogen) is added to the wells. The AChE tracer acts on the Ellman’s Reagent to form a yellow compound.The intensity of the colour, which is determined by spectrophotometry, is proportional to the amount of tracer bound to the well and is inversely proportional to the amount of free rat prolactinpresent in the well during the immunological incubation.

 

rat prolactin ELISA

Related Products