Substance P ELISA
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The Substance P ELISA kit is designed to measure Substance P levels in human, mouse and rat samples.
Overview
Substance P is a neuropeptide that functions as a neurotransmitter and as a neuromodulator, playing a criticial role in pain perception. It is found in the brain and spinal cord, and is associated with inflammatory processes in the joints, causing arthritic pain, as well as low back pain and fibromyalgia. Substance P and other neuropeptides can be released from sensory nerve fibers in the skin, muscle and joints causing a local inflammatory response to certain types of infection or injury.
Data/Specifications
Species: human, mouse, rat
Sample Type: serum, plasma
Sample Preparation: plasma extraction
Sample Size: 50 uL
Standard Curve Range: 0.04 - 25 ng/mL
Sensitivity: 0.04 ng/mL
Assay Length: 4 hrs
Literature/Support
Product Insert:
Substance P Insert (PDF)
Articles/Troublshooting:
Becker, J. B., & Cha, J. H. (1989). Estrous cycle-dependent variation in amphetamine-induced behaviors and striatal dopamine release assessed with microdialysis. Behavioural brain research, 35(2), 117-125.
Malykhina, A. P., Lei, Q., Chang, S., Pan, X. Q., Villamor, A. N., Smith, A. L., & Seftel, A. D. (2013). Bladder outlet obstruction triggers neural plasticity in sensory pathways and contributes to impaired sensitivity in erectile dysfunction. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 304(10), R837-R845.
| References/Citations: | How the Substance P ELISA Kit was used: |
Experimental colitis triggers the release of substance P and calcitonin gene-related peptide in the urinary bladder via TRPV1 signaling pathways. | Measure the concentration of substance P in the urinary bladder of Sprague-Dawley rats. |
Assessment of antidepressant and anxiolytic properties of NK1 antagonists and Substance P in Wistar Kyoto rats | Measure basal plasma and hypothalamic levels of substance P in an animal model of depression - adult male Wistar Kyoto rats and their controls, Wistar rats. |
How It Works
The immunoplate in this kit is pre-coated with secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in sample. The biotinylated peptide is able to interact with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate solution composed of 3,3’,5,5’-tetramethylbenzidine (TMB) and hydrogen peroxide to produce a blue colored solution. The enzyme-substrate reaction is stopped by hydrogen chloride (HCl) and the solution turns to yellow. The intensity of the yellow is directly proportional to the amount of biotinylated peptide-SAHRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide and the peptide in standard solutions or samples to the peptide antibody (primary antibody). A standard curve of a peptide with known concentration can be established accordingly. The peptide with unknown concentration in samples can be determined by extrapolation to this standard curve.
