TNF-alpha ELISA, human

human TNF-alpha ELISA
Catalog Number: 
96 wells

human TNF-alpha (TNFa) ELISA from MD Bioproducts

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For the quantitative determination of TNF-alpha in human serum, plasma and cell culture supernate samples



Tumor necrosis factor alpha (TNF-α) is a potent cytokine with a myriad of innate immune anti-tumor properties. TNF-α has a critical role in the bone and cartilage damage associated with rheumatoid arthritis (RA). TNF-α may be involved in the pathogenesis and/or progression of gestational diabetes mellitus (GDM). TNF-α is expressed in myocardium during compensated pressure-overload hypertrophy and contributes to postischemic myocardial dysfunction. The serum levels of TNF-α were also significantly elevated in active WG (Wegener’s granulomatosis) in the late stages of HIV-associated disease and in the spinal cord of arthritic patients.



Species: human

Sample Type:  serum, plasma, cell culture supernates

Sample Size: 50 uL

Standard Curve Range:  0 - 1 ng/mL

Sensitivity:  0.01 ng/mL

Assay Length:   5 hours



Product Insert:

human TNF-a ELISA Insert (PDF)



ELISA Troubleshooting Guide

ELISA Data Reduction Guide





How It Works

How It Works: 

Human TNF-α ELISA kit is designed for detection of TNF-α in human plasma, serum or cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures TNF-α in less than 5 hours. A murine monoclonal antibody specific for human TNF-α has been pre-coated onto a microplate. TNF-α in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human TNF-α, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


human TNFa ELISA