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Mouse IgG2b anti-Collagen Type II ELISA

Mouse IgG2b anti-Collagen Type II ELISA
Catalog Number: 
M306081
Qty/Size: 
96 wells

OVA-IgE ELISA from MD Bioproducts

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The Collagen Type II (CII) Antibody ELISA kit is used to measure IgG2b antibodies generated against mouse type II collagen used in the induction of arthritis in mice. 

Overview

Overview: 

The Collagen-induced arthritis (CIA) model is one of the most widely used models for the study of Rheumatoid Arthritis (RA). Collagen type II (CII) in adjuvant is used to induce arthritis by triggering autoreactive B cells, which play an important role in the development of arthritis. This B cell response to CII is strictly T cell dependent, where the B cells predominantly switch isotype to IgG. 

 Symbols/Related Terms:
 - COL-2
 - type II collagen antibody ELISA
 - COL2A1
 - pro-alpha 1(II) chain

 

 Symbols/Related Terms:

 - COL-2

 - type II collagen antibody ELISA

 - COL2A1

 - pro-alpha 1(II) chain

 

Data/Specifications

Data/Specifications: 

 

Species: mouse
Sample Type:  serum
Sample Preparation:  1:50
Sample Size: 100 uL
Sensitivity:  0.75 ng/mL. 
Assay Length:   < 3 hrs.

Species: mouse

Sample Type:  serum

Sample Preparation:  1:50

Sample Size: 100 uL

Sensitivity:  0.75 ng/mL. 

Assay Length:   < 3 hrs.

Literature/Support

Literature/Support/Publications: 

How It Works

How It Works: 

ELISA or Enzyme-linked Immunosorbent Assay is a colorimetric based immunoassay utilizing a capture antibody and a detection antibody to provide a unique and powerful assay system. Antibody/antigen reactions take place on the suface of microplate wells that have been previously coated with a monoclonal antibody to mouse IgE heavy chain. Biotinylated ovalbumin and streptavidin-peroxidase, in the presence of substrate quantifies the analyte bound.

 

Assay Principle:

 

ELISA Assay Principle