Rat IgG1 Isotype Control, Low endotoxin, Azide-free
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Rat IgG1 Isotype Control, Low endotoxin, Azide-free antibody
Overview
Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).
Data/Specifications
Ig Isotype:
Rat IgG1k
Specifcity:
Keyhole limpet hemocyanin (KLH)
Form:
This product supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2.
Storage:
Store at 2 – 8 °C
Literature/Support
How To Use
Applications:
- Used for Flow cytometry.
- Used for Enzyme-Linked –Immunosorbent-Assay (ELISA).
- Used for Flourescent-Linked-Immunosorbent-Assay (ELISA).
Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs