MonELISA® Irisin (FNDC5)
MonELISA® Irisin (FNDC5) ELISA is an assay to measure the quantitation of Irisin in human and mouse serum, plasma, cell culture supernatants, and tissue homogenates. This assay provides the most specific results, combined...
MonELISA® Irisin (FNDC5) ELISA is an assay to measure the quantitation of Irisin in human and mouse serum, plasma, cell culture supernatants, and tissue homogenates. This assay provides the most specific results, combined with ease of use and the uniform MonELISA® assay procedure.
Requires MonELISA® Ancillary Reagent Kit, Catalog Number M036080 (sold
Irisin, a skeletal muscle-secreted myokine, is produced in response to physical
exercise. The glycosylated type I membrane protein is released into the bloodstream by cleavage of the fibronectin type III domain-containing protein 5 (FNDC5) triggered by muscle contraction. It contains a N-terminal signal peptide (amino acid (aa) 1-28), a FNIII domain (aa 33-124), a transmembrane domain (aa 150 -170), and a cytoplasmic tail (aa 171-209). The secreted form of FNDC5 contains 112 amino acids (aa 29-140), and a molecular weight of 12 kDa amed irisin. The expression of FNDC5 is under the control of the peroxisome proliferator-activated receptor γ coactivator 1-α, a master regulator of genes involved in metabolism. Irisin is not only present in skeletal muscle, but also in the cardiac muscle, brain, and skin and in smaller amounts in the liver, pancreas, and other tissues. Irisin also plays an important role in central nervous system (CNS) diseases, such as neurodegenerative diseases, cerebral ischaemia and Alzheimer’s disease.
This species-specific MonELISA® Irisin (FNDC5) ELISA is a colorimetric based sandwich immunoassay utilizing an antibody to the specie’s Irisin (FNDC5) bound on the surface of microwells as the capture antibody and a biotinylated antibody to Irisin (FNDC5) as the detection antibody. When antigen is added to the well it is bound by the immobilized antibody. The biotinylated antibody then binds to the antigen forming an antigen-antibody complex. Streptavidin-Peroxidase in the presence of an enzyme substrate quantifies analyte bound. Color development is directly proportional to the antigen concentration in the sample.
- FRCP2 Fibronectin type III repeat-containing protein 2
- Fibronectin Type III Domain-Containing Protein 5
- Fibronectin Type III Domain Containing 5
- Species: Human, Mouse
- Sample Type: Serum, Plasma, Cell Culture Supernatants, Tissue Homogenates
- Sample Size: 100 μL
- Range: 93.75 – 6000 pg/mL
- Sensitivity: < 50 pg/mL
- Assay Length: 2 hrs 40 min