MonELISA® Mouse Total IgE assay is used to measure total IgE levels in mouse serum, plasma, cell culture supernatants and tissue homogenate samples. This assay provides the most specific results, combined with ease of use and the uniform MonELISA® assay procedure.

Requires MonELISA Ancillary Reagent Kit, Catalog Number M036080 (sold separately). 

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IgE is the least abundant isotype but has the capability of triggering powerful immune responses by binding to Fc receptors on the surface of cells such as mast cells, basophils, eosinophils, monocytes, macrophages and platelets.

 IgE is well known for its involvement in eliciting an allergic or asthmatic response. After sensitization with an allergan, through T and B cell signalling stimulates IgE production. IgE is released into the bloodstream where it attaches to receptors on cells such as mast cells and basophils. These cells are then activated upon cross-linking of the IgE with the antigen initiating the allergic cascade.

This species-specific Total IgE Mouse MonELISA® is a colorimetric based sandwich immunoassay utilizing antibody to the specie’s Total IgE bound on the surface of microwells as the capture antibody and a biotinylated antibody to Total IgE as the detection antibody. When antigen is added to the well it is bound by the immobilized antibody. The biotinylated antibody then binds to the antigen forming an antigen-antibody complex. Streptavidin-Peroxidase in the presence of an enzyme substrate quantifies analyte bound.  Color development is directly proportional to the antigen concentration in the sample.