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ELISAs

IL-10 ELISA, human

human IL-10 ELISA
Catalog Number: 
M046049
Qty/Size: 
96 wells

human TNF-alpha (TNFa) ELISA from MD Bioproducts

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For the quantitative determination of IL-10 in human plasma, serum tissue extracts and cell culture supernate samples.

Overview

Overview: 

Interleukin-10 (IL-10) is a regulatory cytokine, and its principal role in vivo is to limit inflammatory response. IL-10 has been shown to influence both the susceptibility and course of various diseases. Monocytes from indeterminate-disease patients display modulatory characteristics related to high IL-10 expression; whereas, monocytes from cardiac-disease patients may be committed to induction of inflammatory responses related to high TNF-α expression. Interleukin 10 (IL-10) is a key cytokine produced by a multitude of immune effector cells and possesses distinct regulatory effects on immune functioning in the skin. The accelerated alveolar bone loss observed in IL-10 (-/-) mice is a late-onset condition and that lack of IL-10 may have an effect on bone homeostasis.

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  serum, plasma, cell culture supernates, tissue extracts

Sample Size: 50 uL

Standard Curve Range:  0.125 - 4 ng/mL

Sensitivity:  0.012 ng/mL

Assay Length:   < 5 hours

Literature/Support

Literature/Support: 

Product Insert:

human IL-10 Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

 

 

 

How It Works

How It Works: 

Human TNF-α ELISA kit is designed for detection of TNF-α in human plasma, serum or cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures TNF-α in less than 5 hours. A murine monoclonal antibody specific for human TNF-α has been pre-coated onto a microplate. TNF-α in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human TNF-α, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

 

human TNFa ELISA