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Aggrecan

Chondroitin Sulphate Neoepitope Antibody

Catalog Number: 
1042014
Qty/Size: 
1.0 mL

chondroitin sulphate neoepitope antibody from MD Bioproducts

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Chondroitin sulfate monoclonal antibody (mouse, clone 1B5) to detect the zero sulphated Chondroitin Sulphate stub neoepitope generated by chondroitinase ABC treatment.

Overview

Overview: 

Recognizes δ unsaturated disaccharide of unsulfated chondroitin generated by chondroitinase ABC digestion.

Data/Specifications

Data/Specifications: 

Immunogen: Bovine nasal cartilage aggrecan that had been deglycosylated with chondroitinase ABC digestion; i.e. BNC (ABC) Core (see Couchman et al 1984 and Caterson et al 1985).

Clone: 1B5

Concentration: 0.1 mg/mL

Isotype: IgG1K

Host: Mouse

Purity: Protein A

Specificity: This antibody recognizes zero sulphated Chondroitin Sulphate stub neoepitope generated by chondroitinase ABC treatment.

Form: Clear liquid; PBS, no preservatives

Storage: -20° C

Literature/Support

Literature/Support/Publications: 

Chondroitin Sulphate Neoepitope Antibody (Insert)

 

A.J. Hayes et al. (2008) Methods 45:10–21
Couchman JR, Caterson B, Christner JB, Baker JR (1984) Mapping by monoclonal antibody 
detection of glycosaminoglycans in connective tissues. Nature 307:650–652
Caterson B, Christner JE, Baker JR, Couchman JR (1985) Production and characterization of monoclonal antibodies directed against connective tissue proteoglycans. Fed Proc 44:386–393
Anthony J. Hayes, Amanda Hall, Liesbeth Brown, Ross Tubo, and Bruce Caterson (2007) Macromolecular Organization and In Vitro Growth Characteristics of Scaffold-free Neocartilage GraftsJournal of Histochemistry & Cytochemistry Volume 55(8): 853–866 

A.J. Hayes et al. (2008) Methods 45:10–21

 

Couchman JR, Caterson B, Christner JB, Baker JR (1984) Mapping by monoclonal antibody detection of glycosaminoglycans in connective tissues. Nature 307:650–652

 

Caterson B, Christner JE, Baker JR, Couchman JR (1985) Production and characterization of monoclonal antibodies directed against connective tissue proteoglycans. Fed Proc 44:386–393

 

Anthony J. Hayes, Amanda Hall, Liesbeth Brown, Ross Tubo, and Bruce Caterson (2007) Macromolecular Organization and In Vitro Growth Characteristics of Scaffold-free Neocartilage GraftsJournal of Histochemistry & Cytochemistry Volume 55(8): 853–866 

How To Use

How To Use: 

Applications:

  • Western Blot: use at dilution of 1/100.
  • Immunohistochemistry: Formalin or paraformaldehyde-fixed paraffin embedded sections, alcohol-fixed frozen sections or un-fixed snap-frozen sections.

Technical Notes:

Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.