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Chondroitin sulfate monoclonal antibody (mouse, clone 1B5) to detect the zero sulphated Chondroitin Sulphate stub neoepitope generated by chondroitinase ABC treatment.
Overview
Overview:
Recognizes δ unsaturated disaccharide of unsulfated chondroitin generated by chondroitinase ABC digestion.
Data/Specifications
Data/Specifications:
Immunogen: Bovine nasal cartilage aggrecan that had been deglycosylated with chondroitinase ABC digestion; i.e. BNC (ABC) Core (see Couchman et al 1984 and Caterson et al 1985).
Clone: 1B5
Concentration: 0.1 mg/mL
Isotype: IgG1K
Host: Mouse
Purity: Protein A
Specificity: This antibody recognizes zero sulphated Chondroitin Sulphate stub neoepitope generated by chondroitinase ABC treatment.
Form: Clear liquid; PBS, no preservatives
Storage: -20° C
How To Use
How To Use:
Applications:
- Western Blot: use at dilution of 1/100.
- Immunohistochemistry: Formalin or paraformaldehyde-fixed paraffin embedded sections, alcohol-fixed frozen sections or un-fixed snap-frozen sections.
Technical Notes:
Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.
