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Keratocan (KERA) monoclonal antibody (mouse, clone Ker-1) to detect keratocan.
Overview
Overview:
Normal keratocan expression in adult tissues is limited to the corneal stroma and is considered a phenotypic marker for keratocytes. In keratocan knockout mice (Kera-/-) the corneal stroma is thinner, the cornea-iris angles are narrower and the collagen fibers of the corneal stroma are disorganized when compared to wild-type animals. In humans, mutations of the keratocan gene (KERA) are associated with the human disease called Cornea plana (CNA2). This disease is characterized by a flattening of the forward convex curvature of the cornea and has been associated with glaucoma. Ultimately, this leads to a decrease in light refraction. Present research is focused on using adult stem cells to regenerate tissue and corneal transparency within knockout mice. Additionally, understanding keratocan in the corneal inflammatory response is a topic of interest.
Related Terms/Symbols:
- KERA
- CNA2
- Keratan sulfate proteoglycans (KSPGs)
- O60938
- KTN
- SLRR2B
Data/Specifications
Data/Specifications:
Clone: Ker-1
Host: Mouse
Isotype: IgM
Purity: Affinity purified on thiophilic column
Form: Liquid, 1 mL/vial
Concentration: 0.1 mg/mL
Storage: Store at -20°C.
Specificity: This Keratocan antibody recognises a protein core epitope in human, bovine, porcine, mouse & chicken tissues.
How To Use
How To Use:
Applications
- ELISA
- Western Blot: recommended dilution 0.4 mg/mL
- IHC
Technical Note
This epitope is best recognized after keratanase and keratanase II treatment/digestion
of section (IHC) or tissue extracts (for Western analyses). In some IHC tissue analyses,
chondroitinase treatment may also be used as this second enzyme treatment facilitates
antibody penetration into the tissue.
