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Keratocan

Keratocan Antibody

Monoclonal Antibody to Keratocan, clone Ker-1
Catalog Number: 
1042008
Qty/Size: 
100 ug

Monoclonal Antibody to Monoclonal Antibody to Keratocan, clone Ker-1 from MD Bioproducts

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Keratocan (KERA) monoclonal antibody (mouse, clone Ker-1) to detect keratocan.

Overview

Overview: 

Normal keratocan expression in adult tissues is limited to the corneal stroma and is considered a phenotypic marker for keratocytes. In keratocan knockout mice (Kera-/-) the corneal stroma is thinner, the cornea-iris angles are narrower and the collagen fibers of the corneal stroma are disorganized when compared to wild-type animals. In humans, mutations of the keratocan gene (KERA) are associated with the human disease called Cornea plana (CNA2). This disease is characterized by a flattening of the forward convex curvature of the cornea and has been associated with glaucoma. Ultimately, this leads to a decrease in light refraction. Present research is focused on using adult stem cells to regenerate tissue and corneal transparency within knockout mice. Additionally, understanding keratocan in the corneal inflammatory response is a topic of interest.


Related Terms/Symbols:

  • KERA
  • CNA2
  • Keratan sulfate proteoglycans (KSPGs)
  • O60938
  • KTN
  • SLRR2B

Data/Specifications

Data/Specifications: 

 

Clone: Ker-1

 

Host: Mouse

 

Isotype: IgM

 

Purity: Affinity purified on thiophilic column

 

Form: Liquid, 1 mL/vial

 

Concentration: 0.1 mg/mL

 

Storage: Store at -20°C. 

 

Specificity: This Keratocan antibody recognises a protein core epitope in human, bovine, porcine, mouse & chicken tissues.

Literature/Support

Literature/Support/Publications: 

Keratocan Antibody Insert (PDF) 

 

Keratocan and lumican play an essential role in corneal transparency of the eye (Blog post - April 19, 2010)

 

 

Immunolocalisation and expression of keratocan in tendon. Rees SG, Waggett AD, Kerr BC, Probert J, Gealy EC, Dent CM, Caterson B, Hughes CE. (2008) Osteoarthritis Cartilage. 
2. Fragmentation of decorin, biglycan, lumican and keratocan is elevated in degener- ate human meniscus, knee and hip articular cartilages compared with age-matched macroscopically normal and control tissues. Melrose J, Fuller ES, Roughley PJ, Smith MM, Kerr B, Hughes CE, Caterson B, Little CB. (2008) Arthritis Res Ther. 10(4):R79. 
3. Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis. Gealy EC, Kerr BC, Young RD, Tudor D, Hayes AJ, Hughes CE, Caterson B, Quantock AJ, Ralphs JR. (2007) Histochem Cell Biol. Dec;128(6):551-5. 

 

Immunolocalisation and expression of keratocan in tendon. Rees SG, Waggett AD, Kerr BC, Probert J, Gealy EC, Dent CM, Caterson B, Hughes CE. (2008) Osteoarthritis Cartilage. 

 

Fragmentation of decorin, biglycan, lumican and keratocan is elevated in degener- ate human meniscus, knee and hip articular cartilages compared with age-matched macroscopically normal and control tissues. Melrose J, Fuller ES, Roughley PJ, Smith MM, Kerr B, Hughes CE, Caterson B, Little CB. (2008) Arthritis Res Ther. 10(4):R79. 

 

Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis. Gealy EC, Kerr BC, Young RD, Tudor D, Hayes AJ, Hughes CE, Caterson B, Quantock AJ, Ralphs JR. (2007) Histochem Cell Biol. Dec;128(6):551-5. 

How To Use

How To Use: 

 

Applications

 

  • ELISA
  • Western Blot: recommended dilution 0.4 mg/mL
  • IHC

 

 

Technical Note

This epitope is best recognized after keratanase and keratanase II treatment/digestion

of section (IHC) or tissue extracts (for Western analyses). In some IHC tissue analyses,

chondroitinase treatment may also be used as this second enzyme treatment facilitates

antibody penetration into the tissue.