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Lumican (LUM) monoclonal antibody (mouse, clone Lum-1) to detect a protein epitope in lumican.
Overview
Overview:
Lumican (LUM) is the major keratan sulfate proteoglycan of the cornea and it binds collagen fibrils. In noncorneal tissues, lumican is present as a low or nonsulfated glycoprotein and has been found in the aorta, cartilage, liver, skeletal muscle, kidney, pancreas, brain, placenta and lung. Complications of the cornea have been confirmed using lumican-null mice. These mice exhibit corneal opacity, skin fragility, and impaired collagen fibrillogenesis. More recent studies show lumican has an integral role in cell migration, adhesion and proliferation. The expression of lumican has also been examined in breast, pancreatic and colorectal cancer. The affect of lumican depends on the type of cancer as it has been shown to have both a positive and negative affect on tumor growth.
Related Terms/Symbols
- LUM
- LDC
- SLRR2D
- P51884
- Lumican proteoglycan
- major keratan sulfate proteoglycan
Data/Specifications
Data/Specifications:
Clone: Lum-1
Host: Mouse
Isotype: IgM
Purity: Affinity purified on thiophilic column
Form: Liquid, 1 mL/vial
Concentration: 0.1 mg/mL
Storage: -20°C.
Specificity: This Lumican antibody recognises a protein epitope in lumican derived from human, bovine, porcine & mouse tissues.
How To Use
How To Use:
Applications
- Western Blot: recommended dilution 0.2 mg/mL
- IHC
Technical Notes
This epitope is best recognized after keratanase and keratanase II treatment/digestion
of section (IHC) or tissue extracts (for Western analyses). In some IHC tissue analyses,
chondroitinase treatment may also be used, as this second enzyme treatment facilitates
antibody penetration into the tissue.
