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MMP

MMP-13 (activated) ELISA

MMP-13 (activated) ELISA
Catalog Number: 
M046015
Qty/Size: 
96 wells

activated MMP-13 ELISA from MD Bioproducts

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The activated MMP-13 ELISA kit is used to measure activated Matrix Metalloproteinase-13 (Collagenase-3) levels in human serum, synovial fluid and cell culture supernates.

Overview

Overview: 

Matrix metalloproteinases (MMPs) comprise a family of secreted and membrane-bound endopeptidases which hydrolyze extracellular matrix proteins. Based on their preferred substrates and on structural features MMPs can be divided into collagenases, gelatinases, stromelysins and membrane-type matrix metalloproteinases. Their activity is tightly regulated by inhibitors. Collagenase 3 (MMP-13) is a secreted 452 residue protein which is released from cells as an inactive zymogen and activated extracellularly by removal of its propeptide. Cleavage of the 84 residue propeptide can be catalysed by other MMPs such as MMP-2 and MMP-14, or by factors like plasmin. Under normal conditions, MMP-13 is expressed during embryogenesis (fetal bone development) and is present only at very low levels in adult tissue. However, this enzyme is reported to be involved in the development and metastasis of breast and lung carcinomas, chondrosarcomas and osteosarcomas, head and neck carcinomas and some forms of skin cancer. Additionally, this enzyme plays an important role in degenerative bone diseases including osteoarthritis and rheumatoid arthritis.

 

 

 

 

Data/Specifications

Data/Specifications: 

Species: Human

Sample Type:  serum, synovial fluid, cell culture supernates

Sample Preparation: Dilute samples

Sample Size: 100 uL

Standard Curve Range:  32-3000 pg/mL

Sensitivity:  7 pg/mL

Assay Length:   4.25 hrs

 

 

Literature/Support

Literature/Support: 

Product Insert:

Activated MMP-13 ELISA Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

 

 

 

How It Works

How It Works: 

The assay provides a highly sensitive and specific quantitative determination of human activated MMP-13 in serum, synovial fluid, and cell culture supernatants. The calibration curve covers the range of 32 pg/mL up to 2000 pg/mL. The sensitivity of the assay is 7 pg/mL.

This quantitative assay is based on a two site sandwich format. A monoclonal antibody specific for the activated form of MMP-13 is immobilized on the plate. MMP-13 will be bound to the wells, other components of the sample are removed by aspiration and washing of the plate. The analyte is detected by two steps using a secondary biotin labelled antibody and a highly polymerised streptavidin-peroxidase conjugate. Any excess is removed by aspiration and washing after each detection step. The amount of peroxidase bound to each well is determined by the addition of TMB substrate. The reaction is stopped by adding the stop solution, and the resultant color is read in a microtiter plate reader at 450 nm. The concen- tration of activated MMP-13 in a sample is determined by interpolation from the standard curve. One kit contains reagents for 96 determinations, thus allowing the mea- surement of one standard curve and 40 samples in duplicate.

 

activated MMP-13 ELISA