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Prolactin

Prolactin (mouse) ELISA

mouse prolactin ELISA
Catalog Number: 
M046041
Qty/Size: 
96 wells

mouse prolactin ELISA from MD Bioproducts

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The Prolactin (PRL) ELISA kit is designed to measure Mouse Prolactin levels in serum and plasma.

Overview

Overview: 

Prolactin (PRL) or luteotropic hormone (LTH) is a peptide hormone with a molecular weight of 24,000 daltons and a structure that is similar to that of growth hormone and placental lactogen. It is primarily associated with pregnancy and lactation and is expressed in the anterior pituitary, uterus and placenta. During pregnancy, high circulating concentrations of estrogen promote prolactin production. The resulting high levels of prolactin secretion cause further maturation of the mammary glands, preparing them for lactation. 

Data/Specifications

Data/Specifications: 

Species: mouse

Sample Type:   cell culture media

Sample Size: 50 uL

Standard Curve Range:  0.39 - 50 ng/mL

Sensitivity:  0.2 ng/mL

Assay Length:   overnight incubation

Literature/Support

Literature/Support: 

Product Insert:

Mouse Prolactin ELISA Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

 

 

 

How It Works

How It Works: 

This Enzyme Immunoassay (EIA) is based on the competition between unlabelled mouse prolactin and acetylcholinesterase (AChE) linked to mouse prolactin (tracer) for limited specific rabbit anti-mouse prolactin antiserum sites. The complex rabbit antiserum-mouse prolactin (free prolactin or tracer) binds to the mouse monoclonal anti-rabbit antibody that is attached to the well. The plate is washed to eliminate the unbound tracer and Ellman’s reagent (enzymatic substrate for AChE and chromogen) is added to the wells. The AChE tracer acts on the Ellman’s reagent to form a yellow compound. The intensity of the color is determined by spectrophotometry and is inversely proportional to the amount of free mouse prolactin present in the well during the immunological incubation.

 

mouse prolactin ELISA

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