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Isotype controls are most commonly used in flow cytometry and immunohistochemistry experiments as negative controls that measure the level of non-specific background signal caused by primary antibodies. Background signal is due to Igs binding to non-specifcally to Fc receptors present on a cell surface (ex. mice antibodies binding to human leukocytes non-specifically in which investigators would want to use a mouse isotype control when working with human cells or tissues).
Data/Specifications
Data/Specifications:
Ig Isotype: Rat IgG1k
Specifcity: Keyhole limpet hemocyanin (KLH)
Form: The R-Phycoerthrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL PBS/NaN3
Used for Flow cytometry: working dilution ≤ 1 ug / 106 cells
Note: Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs
