Glycoprotein/Proteoglycan

Beta-2-Microglobulin ELISA, human

human Beta-2-Microglobulin ELISA For the quantitative determination of Beta-2-Mi
Catalog Number: 
M046036
Qty/Size: 
96 wells

human beta-2-microglobulin ELISA from MD Bioproducts

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For the quantitative determination of Beta-2-Microglobulin in human plasma, serum and cell culture supernate samples

Overview

Overview: 

β2-Microglobulin (β2M) is a small serum protein that constitutes the light chain of the major histocompatibility class I human leukocyte antigen (HLA class I), an integral membrane protein involved in the immune response. The protein is 99 amino acid residues in length and has molecular mass of 12 kDa. β2M is released from the cell surface of HLA class I into the serum and carried to the kidneys for degradation and secretion. In chronic renal failure, β2M accumulates as insoluble amyloid aggregates and causes arthralgias, destructive osteoarthropathies, carpal tunnel syndrome and dialysis-related amyloidosis. Elevated serum β2M levels are associated with poor prognosis in multiple myeloma and lymphoma.

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  serum, plasma, cell culture supernate

Sample Size: 50 uL

Standard Curve Range:  0 - 200 ng/mL

Sensitivity:  0.7 ng/mL

Assay Length:   3 hours 40 min

Literature/Support

Literature/Support: 

How It Works

How It Works: 

Human β2-Microglobulin ELISA kit is designed for detection of human β2-Microglobulin in plasma, serum, urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures β2-Microglobulin in 4 hours. A polyclonal antibody specific for β 2-Microglobulin has been pre-coated onto a microplate. β2-Microglobulin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for β2-Microglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

 

principle of assay for beta-2-microglobulin ELISA

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