Description: Purified Rat Collagen Type I (Atelocollagen) from rat tail tendon
Form: Lyophilized, salt free, 30 mg/ vial
Sterility: Passed sterility test for bacteria and fungi
Source: Rat tail tendons
Purity: Rat collagen Type I > 90% / Rat collagen type III <10%
Purification: Collagen was extracted from dissected tissue into diluted acetic acid after mild pepsin treatment. Collagen type I was purified by using differential salt precipitation.
Reconstitution: Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8 °C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspensions). Recommended concentration 1-5 mg/mL.
Storage: Collagen dissolved in acetic acid is stable at 4°C for 1 month. Lyophilized collagen long term storage at -20°C or lower.
Applications: Cell culture (coating or 3D gel)