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Aggrecan Antibody, N-terminal neoepitope DIPEN

Catalog Number: 
1042002
Qty/Size: 
100 ug

monoclonal antibody to aggrecan N-terminal epitope DIPEN, clone BC-4 from MD Bioproducts

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International: +41-44 986 2628

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Aggrecan monoclonal antibody to N-terminal neoepitope DIPEN (mouse, clone BC-4).

 

Overview

Overview: 

Proteoglycans are categoried depending upon the nature of their glycosaminoglycan chains (chondroitin sulfate, dermatan sulfate, heparan sulphate and keratan sullfate) as well as characterized by size. 

Aggrecan is a large aggregating proteoglycan of articular cartilage. It is found also in aorta tissue, discs, tendons and in the perineuronal net. It is responsible for hydrating cartilage, giving it compressibility and resiliance during joint loading, thereby playing a major role in the normal function of cartilage. Depletion of glycosaminoglycan bearing aggrecan fragments is one of the earliest events in cartilage destruction. 

 

A) Aggrecan monomer showing proteolytic cleavage sites

B) Aggregate showing aggrecan monomers bound to hyaluronic acid

 

Aggrecan

Data/Specifications

Data/Specifications: 

Immunogen: DIPEN synthetic peptide conjugate.

 

Clone: BC-4

 

Host: Mouse

 

Myeloma: x63-Ag8.653

 

Isotype: IgG1

 

Light Chain type: kappa

 

Specificity: Recognizes the N-terminal neoepitope sequence (...DIPEN) generated at the “MMP cleavage site” after MMP catabolism in the interglobular domain of aggrecan between amino acids ..PEN341 and 342FFG.

 

Cross-reactivity: This antibody cross-reacts with human, Rat, guinea pig, horse, and Pig.

 

Purity: Affinity purified on protein G

 

Form: Liquid, 1 mL/vial

 

Concentration: 0.1 mg/mL

 

Storage: -20° C

Literature/Support

Literature/Support: 

Aggrecan Antibody, clone BC4 Insert (PDF, 332 KB)


Joint Disease and Aggrecan (blog post)

 

References/Citations:How the Aggrecan N-terminal Neoepitope Antibody was used:

Recapitulation of endochondrial bone formation using human adult mesenchymal stem cells as a paradigm for developmental engineering
Celeste Scotti et al., PNAS, April 2010; 107:7251-7256.

(Supporting Literature:  Supporting Literature )

Immunohistochemistry analysis to characterize the extracellular matrix of late hypertrophic cartilage implants harvested from mice.
Identification of a Novel HtrA1-susceptible Cleavage Site in Human Aggrecan: EVIDENCE FOR THE INVOLVEMENT OF HtrA1 IN AGGRECAN PROTEOLYSIS IN VIVO
Angela Chamberland et al.,  J. Biol. Chem., Oct 2009; 284: 27352 - 27359.
Western blot analysis to detect the N-terminal neoepitope-DIPEN cleavage site of purified full-length human aggrecan, after digestion with MMP-13. This was visualized at ~50 kDa.


 



 

How To Use

How To Use: 

Applications: 

 

  • Western-Blotting - Suggested dilution: 1:100 Detects a a band of approximately 60 kDa
  • ELISA
  • IHC

 

 

Technical Notes:

This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded

sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections.

Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01

Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10μg S-GAG

of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry

(1, 2).