Aggrecan IGD Antibody
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Monoclonal Antibody to IGD Aggrecan, clone 6B4. This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples
Overview
Proteoglycans are categoried depending upon the nature of their glycosaminoglycan chains (chondroitin sulfate, dermatan sulfate, heparan sulphate and keratan sullfate) as well as characterized by size.
Aggrecan is a large aggregating proteoglycan of articular cartilage. It is found also in aorta tissue, discs, tendons and in the perineuronal net. It is responsible for hydrating cartilage, giving it compressibility and resiliance during joint loading, thereby playing a major role in the normal function of cartilage. Depletion of glycosaminoglycan bearing aggrecan fragments is one of the earliest events in cartilage destruction.
A) Aggrecan monomer showing proteolytic cleavage sites
B) Aggregate showing aggrecan monomers bound to hyaluronic acid
Data/Specifications
Immunogen: Synthetic peptide: EPEEPFTFAPEI
Clone: 6B4
Host: Mouse
Myeloma: x63-Ag8.653
Isotype: IgG1
Light Chain type: kappa
Specificity: Recognizes the linear amino acid sequence 394EPEEPFTFAPEI406 present in the interglobular domain (IGD) of human and bovine aggrecan.
Cross-reactivity: This antibody cross-reacts with human and bovine
Purity: Affinity purified on protein G
Form: Liquid, 1 mL/vial
Concentration: 0.1 mg/mL
Storage: -20° C
Literature/Support
Aggrecan IGD Antibody Insert (PDF, 334 KB)
How To Use
Applications
- ELISA: Use at an assay dependant dilution.
- IHC-P: Use at an assay dependant dilution.
- IHC-Fr: Use at an assay dependant dilution.
- WB: Use at a dilution of 1/100.
Technical Notes
Optimal dilutions/concentrations should be determined by the end user. This antibody
should work in IHC on formalin or paraformaldehyde-fixed paraffin embedded sections as
well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections.
Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01
Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG
of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.