Collagen Antibody Induced Arthritis

A rapid and synchronized alternative to the CIA and K/BxN models that reduces variability, eliminates the need for expensive colonies and conserves test compound, controls and vivarium space.

The Collagen Antibody-Induced Arthritis model (CAIA) using ArthritoMab™ Antibody Cocktail is ideal for rapidly screening and evaluating anti-inflammatory therapeutic agents. It is also relevant for studying inflammatory mediators such as cytokines, chemokines, matrix metalloproteinases, and other factors such as the role of bacterial flora and their by-products in triggering and exacerbating arthritis.

Collagen-induced arthritis (CIA) in mice is widely used as an experimental model for rheumatoid arthritis (RA) in humans. CIA is mediated by autoantibodies, which bind to a particular region of type II collagen (CII). The ability to induce arthritis using this arthritogenic antibody cocktail provides an efficient protocol for the induction of antibody-mediated arthritis that can be used as a shorter, more synchronized alternative to the CIA model and the K/BxN model.

 

Benefits of the CAIA model

  • Length of study: Arthritis develops in mice typically within 24-48 hrs. allowing the completion of a study within 2 weeks reducing the number of assessments and scoring periods.
  • Reduced group size: The rate of incidence is nearly 100% depending on the strain allowing for smaller group sizes.
  • Synchronization: The onset of disease is synchronized between animals simplifying treatment schedules.
  • Susceptibility: Arthritis is induced not only in CIA-susceptible DBA/1 and B10.RIII mice, but also in some CIA-resistant mice, such as Balb/c as well C57/bl6.
  • Eliminates expensive colonies: Models such as the K/BxN serum transfer model require labs to maintain expensive colonies and the sera can vary from batch to batch.

 

Comparison

 

ArthritoMab™

Competitor

Epitopes Recognized

CB11, CB10, CB8

CB11 only

Disease Progression

Steady & Controlled

Rapid & Severe

Paw involvement

Consistent & Predominantely rear

Variable & unpredictable

Animals/vial

25

20

   Collagen Antibody-Induced Arthritis using ArthritoMab Antibody Cocktail

The Collagen Antibody Induced Arthritis (CAIA) model is a relevant model for studying the efferent phase of RA, where leukocytes are attracted and respond to the immune complex in the joint. It is induced using a cocktail of antibodies to anti-CII and contains pathogenic features similar to that of RA such as pannus formation, cellular infiltration, synovitis and cartilage/bone destruction.

Download the whitepaper

 

Antibody Cocktail

ArthritoMab Antibody Cocktail for Balb/c, DBA/1, R10.RIII, MD Bioproducts
ArthritoMab™ Antibody Cocktail for Balb/c, DBA/1, R10.R111
SKU: CIA-MAB-50
Size: 50mg
ArthritoMab™ Arthritis Inducing Antibody Cocktail is a cocktail of 4 arthritogenic monoclonal antibodies to collagen II (CII) used for inducing arthritis in the anti-Collagen Antibody Induced Arthritis (CAIA) model. It is an excellent alternative to both the K/BxN and CIA models. Induction is rapid and results in a synchronized, steady and controlled disease progression that exhibits histological similarities to the classic CIA model. 
ArthritoMab Antibody Cocktail for C57BL/6, TG
ArthritoMab™ Antibody Cocktail for C57BL/, TG
SKU: CIA-MAB-2C
Size: 50mg
ArthritoMab™ Antibody Cocktail is a reformulated cocktail of 4 monoclonal antibodies for the induction of arthritis as an alternative to the widely used collagen-induced arthritis (CIA) model. Many transgenic strains of mice are on a C57BL/6 background. However this strain is refractory to arthritis induction either by CIA or CAIA. Traditionally to overcome this in the CAIA model an increased dose of antibody cocktail is given. This reformulated cocktail of antibodies is optimized for production of arthritis in C57BL/6, inducing arthritis with lower doses of antibody. This new formulation can also be used in other strains of mouse, which traditionally require greater amounts of cocktail.

 

Abreviations:

  • Anti-Collagen Induced arthritis (ACIA)
  • Monoclonal Antibody induced arthritis (mAb-RA)
  • Collagen Antibody Induced Arthritis (CAIA)