Chondroitinase generated C-4-S & DS Antibody
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Chondroitin-4-sulfate (C-4-S) monoclonal antibody that's also specific to dermatan-sulfate (DS) neoepitope (mouse, clone 2B6).
Overview
Monoclonal antibody to delta-unsaturated glucuronic acid adjacent to N-acetylgalactosamine- 4-sulphate in the non-reducing terminal dissacharide “stub” of 4-sulphated chondroitin sulfate that is produced after chondroitinase digestion of chondroitin-4- sulphate glycosaminoglycan chains (chondroitinase ABC or ACII) or dermatan sulphate glycosaminoglycan chains (chondroitinase ABC or chondroitinase B).
Related Terms/Symbols:
- Chondroitin-4-sulfate
- C-4-S
- Dermatan-sulfate
Data/Specifications
Immunogen: Bovine nasal cartilage aggrecan that had been deglycosylated with chondroitinase ABC digestion; i.e. BNC (ABC) Core (see Couchman et al 1984 and Caterson et al 1985).
Clone: 2B6
Isotype: IgG
Host: Mouse
Purity: Affinity purified
Specificity: Chondroitin-4-sulphate (C-4-S) after treatment with Chondroitinase ABC or ACII. Dermatan sulphate (DS) neoepitope after treatment with Chondroitinase ABC or B.
Form: liquid
Storage: -20° C
Literature/Support
Antibody to Chondroitinase-generated C-4-S and DS neoepitope Insert (PDF, 338 KB)
Joint Disease and Aggrecan (blog post)
How To Use
Applications
- ELISA
- Western Blot
- Immunohistochemistry
Technical Notes
Pretreatment with Chondroitinase required. 1x10-3 U per 10 mg GAG at 37 °C for 1 hour.