Collagen Type I Antibody, anti-rat
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Collagen type I polyclonal antibody (rabbit anti-rat) purified from rabbits injected with type I collagen that was extracted/purified from rat skin.
Overview
Type I collagen is the most abundant form of collagen in the human body and is synthesized mainly by fibroblasts, osteoblasts, odontoblasts and chondroblasts. It is located in the extracellular matrix of many tissues of the body including cartilage, bone, tendon, skin and the sclera of the eye. Type I collagen is composed of two pro-α1(I) chains, produced from the COL1A1 gene, and one pro-α2(I) chain, produced from the COL1A2 gene. Mutations in the genes that produce collagen type I are responsible for causing various health conditions including Ehlers-Danlos syndrome, osteogenesis imperfecta, osteoporosis and Caffey disease.
Data/Specifications
Format: IgG fraction, freeze-dried (0.5 ml). Reconstitute with 0.5 ml di H2O and store aliquots at -20°C.
Specificity (% at 1:200 RIA dilution):
Rat Collagen, Type I : 100%
Rat Collagen, Types II, III, V : < 0.1%
Human, mouse, chicken Collagen Type I: < 0.1%
Rat elastin: <0.1%
Literature/Support
Anti-Rat Collagen Type I Antibody Insert (PDF, 283 KB)
How To Use
For immunostaining of extra or intracellular components in light microscopy and the quantitation of Collagen Type I in biological fluids or in culture supernatants.
Applications
- IFA
- IHC(p)
- (SP) RIA
- ELISA
Working Dilutions
- Indirect IFA (with fluoresceine anti-rabbit IgG conjugate): >1:80 on frozen rat tissues (liver, skin...).
- IHC(p): >1:500 on fixed, paraffin-embedded rat tissues
- ELISA: >1:200 (OD <500)