Collagen Type I Antibody, anti-rat

Anti-rat Collagen Type I Antibody
Catalog Number: 
203004
Qty/Size: 
0.5 mL

Anti-rat Collagen Type I Antibody from MD Bioproducts

North America: 1-888-USMDBIO
International: +41-44 986 2628

Or you can order by using our online form
Have questions or need bulk? No problem, just ask.

Collagen type I polyclonal antibody (rabbit anti-rat) purified from rabbits injected with type I collagen that was extracted/purified from rat skin.

 

Overview

Overview: 

Type I collagen is the most abundant form of collagen in the human body and is synthesized mainly by fibroblasts, osteoblasts, odontoblasts and chondroblasts. It is located in the extracellular matrix of many tissues of the body including cartilage, bone, tendon, skin and the sclera of the eye. Type I collagen is composed of two pro-α1(I) chains, produced from the COL1A1 gene, and one pro-α2(I) chain, produced from the COL1A2 gene. Mutations in the genes that produce collagen type I are responsible for causing various health conditions including Ehlers-Danlos syndrome, osteogenesis imperfecta, osteoporosis and Caffey disease.

Data/Specifications

Data/Specifications: 

Format: IgG fraction, freeze-dried (0.5 ml). Reconstitute with 0.5 ml di H2O and store aliquots at -20°C.

 

Specificity (% at 1:200 RIA dilution):

Rat Collagen, Type I : 100%

Rat Collagen, Types II, III, V : < 0.1%

Human, mouse, chicken Collagen Type I: < 0.1%

Rat elastin: <0.1%

 

 

Literature/Support

Literature/Support: 

How To Use

How To Use: 

For immunostaining of extra or intracellular components in light microscopy and the quantitation of Collagen Type I in biological fluids or in culture supernatants.

 

Applications

  • IFA
  • IHC(p)
  • (SP) RIA
  • ELISA

 

Working Dilutions

  • Indirect IFA (with fluoresceine anti-rabbit IgG conjugate): >1:80 on frozen rat tissues (liver, skin...).
  • IHC(p): >1:500 on fixed, paraffin-embedded rat tissues
  • ELISA: >1:200 (OD <500)