PCR Mycoplasma Detection
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The PCR Mycoplasma test kit provides a rapid and sensitive PCR method for monitoring your cell cultures for mycoplasma contamination.
Overview
Cell Cultures are used every day as a part of research methods. You rely on these cultures to be healthy, yet up to 35% may be contaminated due to mycoplasma. Mycoplasma contamination affects many cell functions including metabolism, morphology, protein synthesis and cell proliferation - all of which lead to unreliable results as well as lost time and resources. Since contamination is not typically visible by turbidity, routine screening for mycoplasma contamination is essential.
Data/Specifications
The PCR Mycoplasma Test Kit is adequate to diagnose cell cultures infected with mycoplasmas. Infections usually result in mycoplasma titers of 105 –108 CFU/mL
The kit allows detection of Acholeplasma and Spiroplasma and the following Mycoplasma species:
- M. fermentans
- M. hyorhinis
- M. arginini
- M. orale
- M. salivarium
- M. hominis
- M. pulmonis
- M. arthritidis
- M. bovis
- M. pneumoniae
- M. pirum
- M. capricolum
Sensitivity
| Type of Mycoplasma | Minimum Concentration Detected |
| M. FERMENTANS | 240 CFU/mL |
| M. CAPRICOLOM | 110 CFU/mL |
| PENETRANS | 200 CFU/mL |
| HYORHINIS | 210 CRU/mL |
Literature/Support
PCR Mycoplasma Test Kit Insert (PDF, 388 KB)
PCR Mycoplasma Test Kit MSDS (PDF)
References/Citations:
T cell, IL-12, and IFN- Driven Viral Clearance in Measles Virus-Infected Brain Tissue
Samantha, R et al., J. Virol., Jan 2011
Recruitment and Endo-Lysosomal Activation of TLR9 in Dendritic Cells Infected with Trypanosoma cruzi
Daniella C. Bartholomeu et al., J. Immunol., Jul 2008; 181: 1333 - 1344.
Senescence-induced alterations of laminin chain expression modulate tumorigenicity of prostate cancer cells.
Sprenger CC, et al. Neoplasia. 2008 Dec; 10(12):1350-61.
How It Works
Principle
rRNA gene sequences of prokaryotes, including mycoplasmas, are well conserved, whereas, the lengths and sequences of the spacer region in the rRNA operon (the region between 16S and 23S gene, for example) differ from species to species. The detection procedure utilizing the PCR process with this primer set consists of:
- Amplification of a conserved and mycoplasma-specific 16S rRNA gene region using two primers.
- Detection of the amplified fragment by agarose gel electrophoresis.
This system does not allow the amplification of DNA originating from other sources such as cultured cells or bacteria, which affect the detection result. Amplification of the gene sequence with PCR using this primer set enhances not only the sensitivity, but also the specificity of detection. Amplified products are then detected by agarose gel electrophoresis.