ACTH ELISA
Please register to view price.
Please inquire about bulk pricing.
Adrenocorticotropic hormone (ACTH) ELISA kit to measure ACTH levels in human, mouse and rat plasma samples.
Overview
ACTH (Adrenocorticotropic hormone) or corticotropin is a 39-amino acid peptide hormone (MW=4500) secreted by the pituitary to regulate the production of steroid hormones by the adrenal cortex. ACTH secretion from the anterior pituitary is controlled by both a classical negative feedback control mechanism and CNS-stress mediated control system. Various types of stress or pain perceived in higher levels of the brain modulate secretion of the hypothalamic neurosecretory hormone, corticotropin releasing hormone (CRH), a 41-amino acid peptide. CRH stimulates pituitary ACTH secretion. The second peptide that modulates ACTH secretion is vasopressin (AVP). AVP secretion is also stimulated by stress and acts synergistically with CRH to increase ACTH secretion in the pituitary portal circulation. ACTH increases the synthesis and release of all adrenal sterioids, aldosterone, cortisol and adrenal androgens. It is the principal modulator of cortisol, the most important glucocorticoid in man. As the cortisol level in blood increases, release of ACTH is inhibited directly at the pituitary level. Through this same mechanism, decreasing cortisol levels lead to elevated ACTH levels.
Biologically active ACTH results from enzymatic cleavage of a large precursor molecule, pro-opiomelanocortin (POMC). This molecule contains within its structure the amino acid sequences of ACTH, Pro-ACTH, -melanocyte stimulating hormone, lipotropin, as well as endorphin and the enkephalins. Because the reaction in immunoassays is determined by antigenic structure, not biological function, the usual ACTH RIA reacts with POMC, Pro-ACTH, ACTH and some fragments of the ACTH.
Data/Specifications
Species: human, mouse, rat
Sample Type: plasma
Sample Size: 200 uL
Standard Curve Range: 0 - 500 pg/mL
Sensitivity: 0.46 pg/mL
Assay Length: 4.5 hours
Literature/Support
ACTH ELISA Insert (PDF)
Stress and Autoimmune Disease: the role of ACTH (blog post)
| References/Citations | How the ACTH ELISA was used: |
| The hypothermic response to bacterial lipopolysaccharide critically depends on brain CB1, but not CB2 or TRPV1, receptors Alexandre, A.S. et al., (2011) J Physiol. 589:2415 | Measure the concentration of ACTH in plasma obtained from control rats (saline) and LPS-induced hyperthermia rats. |
| Thymocyte-Synthesized Glucocorticoids Play a Role in Thymocyte Homeostasis and Are Down-Regulated by Adrenocorticotropic Hormone Shengjun Qiao et al., Endocrinology, Sep 2009; 150: 4163 - 4169. | Measure the concentration of ACTH in sera obtained from C57BL/6 wild-type mice and genetically modified mice. |
| Plasma Osteopontin Modulates Chronic Restraint Stress-Induced Thymus Atrophy by Regulating Stress Hormones: Inhibition by an Anti-Osteopontin Monoclonal Antibody Kathryn X. Wang et al., J. Immunol., Feb 2009; 182: 2485 - 2491. | Measure the level of ACTH in plasma obtained from wild-type and knock-out BALB/c mice under chronic restraint stress conditions. |
| Complex Role of the Mitochondrial Targeting Signal in the Function of Steroidogenic Acute Regulatory Protein Revealed by Bacterial Artificial Chromosome Transgenesis in Vivo Goro Sasaki et al., Mol. Endocrinol., Apr 2008; 22: 951 - 964. | Measure plasma ACTH levels of 8-wk old mice of different genotypes. Blood was collected by cardiac puncture and ACTH was measured in pg/ml units. |
Inhibition of brain proinflammatory cytokine synthesis reduces hypothalamic excitation in rats with ischemia-induced heart failure | Measure plasma ACTH levels in Sprague-Dawley rats. Rats were subject to vehicle treatment in heart failure vs SHAM models. Blood was collected in chilled EDTA tubes, separated and stored at -80C. |
| Electroacupuncture activates corticotrophin-releasing hormone-containing neurons in the paraventricular nucleus of the hypothalammus to alleviate edema in a rat model of inflammation Aihui Li, et al. BMC Complement Altern Med. 2008; 8:20 | Measure plasma ACTH levels in male Sprague-Dawley rats. Plasma was prepared from blood centrifuged at (1310 g) for 15 minutes at 4 C, collected and stored at -80 C. Rats underwent various experimental treatments including electroacupuncture and inflammation. |
| Lesions of the anteroventral third ventricle region exaggerate neuroendocrine and thermogenic but not behavioral responses to a novel environment Douglas G. Whyte and Alan Kim Johnson Am J Physiol Regulatory Integrative Comp Physiol, Jan 2007; 292: R137 - R142. | Measure plasma ACTH levels of male Sprague Dawley rats. Blood was collected in EDTA collection tubes, centrifuged at 1,000 g for 15 minutes at 4C, collected and stored at -80C. Rats were studied in SHAM and anteroventral third ventricle (AV3V) ablation models. |
| Neuroendocrine profiling in inherited stress-induced arterial hypertension rat strain with stress-sensitive arterial hypertension A L Markel et al., J. Endocrinol., Dec 2007; 195: 439 - 450. | Measure plasma ACTH levels in rats. Blood was collected in ice-cold EDTA-coated tubes, centrifuge and collected for storage at -70C. Rats investigated were ISIAH and WAG strains under stress conditions. |
| The Effects of SOM230 on Cell Proliferation and Adrenocorticotropin Secretion in Human Corticotroph Pituitary Adenomas Dalia L. Batista et al., J. Clin. Endocrinol. Metab., Nov 2006; 91: 4482 - 4488. | Measure ACTH levels in media from the primary cell culture of human corticotroph adenomas obtained from Cushing's disease patients that underwent transsphenoidal surgery (tumor samples). |