Mouse OVA-IgE ELISA

The Mouse OVA IgE 96-well ELISA Kit is used to measure OVA specific IgE levels in mouse serum and cell culture supernates. Assay time < 2.5 hours.    IgE is the...

ELISA

M036005N

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For research use only. Not for use in diagnostic procedures.

The Mouse OVA IgE 96-well ELISA Kit is used to measure OVA specific IgE levels in mouse serum and cell culture supernates. Assay time < 2.5 hours. 

 

IgE is the least abundant isotype but has the capability of triggering powerful immune responses by binding to Fc receptors on the surface of cells such as mast cells, basophils, eosinophils, monocytes, macrophages and platelets.

IgE is well known for its involvement in eliciting an allergic or asthmatic response. Ovalbumin (OVA) is widely used for inducing an allergic response in mouse models of allergic asthma. Through T and B cell signalling, IgE production is stimulated and released into the bloodstream where it attaches to receptors on cells such as mast cells and basophils. These cells are then activated upon cross-linking of the IgE with the antigen initiating the allergic cascade.

References/Citations:

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Paez-Cortez, J., Krishnan, R., Arno, A., Aven, L., Ram-Mohan, S., Patel, K. R., & Fine, A. (2013). A new approach for the study of lung smooth muscle phenotypes and its application in a murine model of allergic airway inflammation.

Cloots, R. H., Sankaranarayanan, S., de Theije, C. C., Poynter, M. E., Terwindt, E., van Dijk, P., & Koehler, S. E. (2013). Ablation of Arg1 in hematopoietic cells improves respiratory function of lung parenchyma, but not that of larger airways or inflammation in asthmatic mice. American Journal of Physiology-Lung Cellular and Molecular Physiology, 305(5), L364-L376.

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McKee, A. S., Burchill, M. A., Munks, M. W., Jin, L., Kappler, J. W., Friedman, R. S., & Marrack, P. (2013). Host DNA released in response to aluminum adjuvant enhances MHC class II-mediated antigen presentation and prolongs CD4 T-cell interactions with dendritic cells. Proceedings of the National Academy of Sciences, 110(12), E1122-E1131.

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Lombardi, V., Speak, A. O., Kerzerho, J., Szely, N., & Akbari, O. (2012). CD8α&plus; β− and CD8α&plus; β&plus; plasmacytoid dendritic cells induce Foxp3&plus; regulatory T cells and prevent the induction of airway hyper-reactivity. Mucosal immunology, 5(4), 432-443.

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MacSharry, J., O'Mahony, C., Shalaby, K. H., Sheil, B., Karmouty-Quintana, H., Shanahan, F., & Martin, J. G. (2012). Immunomodulatory effects of feeding with Bifidobacterium longum on allergen-induced lung inflammation in the mouse. Pulmonary Pharmacology & Therapeutics, 25(4), 325-334.

Kim, S. R., Lee, K. S., Lee, K. B., & Lee, Y. C. (2012). Recombinant IGFBP‐3 inhibits allergic lung inflammation, VEGF production, and vascular leak in a mouse model of asthma. Allergy, 67(7), 869-877.

Emo, J., Meednu, N., Chapman, T. J., Rezaee, F., Balys, M., Randall, T., ... & Georas, S. N. (2012). Lpa2 is a negative regulator of both dendritic cell activation and murine models of allergic lung inflammation. The Journal of Immunology, 188(8), 3784-3790.

Diesner, S. C., Olivera, A., Dillahunt, S., Schultz, C., Watzlawek, T., Förster-Waldl, E., ... & Rivera, J. (2012). Sphingosine-kinase 1 and 2 contribute to oral sensitization and effector phase in a mouse model of food allergy. Immunology letters, 141(2), 210-219.

Carr, V. M., Robinson, A. M., & Kern, R. C. (2012). Tissue-specific effects of allergic rhinitis in mouse nasal epithelia. Chemical senses, bjs048.

Singh, S. P., Gundavarapu, S., Peña-Philippides, J. C., Mishra, N. C., Wilder, J. A., Langley, R. J., ... & Sopori, M. L. (2011). Prenatal secondhand cigarette smoke promotes Th2 polarization and impairs goblet cell differentiation and airway mucus formation. The Journal of Immunology, 187(9), 4542-4552.

Reisacher, W. R., Liotta, D., Yazdi, S., & Putnam, D. (2011, September). Desensitizing mice to ovalbumin through subcutaneous microsphere immunotherapy (SMITH). In International forum of allergy & rhinology (Vol. 1, No. 5, pp. 390-395). Wiley Subscription Services, Inc., A Wiley Company.

MacNeil, A. J., Yang, Y. J., & Lin, T. J. (2011). MAPK Kinase 3 Specifically Regulates FcεRI-Mediated IL-4 Production by Mast Cells. The Journal of Immunology, 187(6), 3374-3382.

Stemmy, E. J., Balsley, M. A., Jurjus, R. A., Damsker, J. M., Bukrinsky, M. I., & Constant, S. L. (2011). Blocking cyclophilins in the chronic phase of asthma reduces the persistence of leukocytes and disease reactivation. American journal of respiratory cell and molecular biology, 45(5), 991-998.

Kim, S. R., Lee, K. S., Park, S. J., Min, K. H., Lee, M. H., Lee, K. A., & Lee, Y. C. (2011). A novel dithiol amide CB3 attenuates allergic airway disease through negative regulation of p38 mitogen-activated protein kinase. American journal of respiratory and critical care medicine, 183(8), 1015-1024.

Wang, W., Zhu, Z., Zhu, B., & Ma, Z. (2011). Peroxisome Proliferator-Activated Receptor–γ Agonist Induces Regulatory T Cells in a Murine Model of Allergic Rhinitis. Otolaryngology--Head and Neck Surgery, 144(4), 506-513.

Balsley, M. A., Malesevic, M., Stemmy, E. J., Gigley, J., Jurjus, R. A., Herzog, D., & Constant, S. L. (2010). A cell-impermeable cyclosporine A derivative reduces pathology in a mouse model of allergic lung inflammation. The Journal of Immunology, 185(12), 7663-7670.

Lauenstein, H. D., Quarcoo, D., Plappert, L., Schleh, C., Nassimi, M., Pilzner, C. & Groneberg, D. A. (2011). Pituitary adenylate cyclase‐activating peptide receptor 1 mediates anti‐inflammatory effects in allergic airway inflammation in mice. Clinical & Experimental Allergy, 41(4), 592-601.

Kim, S. R., Lee, K. S., Park, S. J., Min, K. H., Lee, M. H., Lee, K. A., ... & Lee, Y. C. (2011). A novel dithiol amide CB3 attenuates allergic airway disease through negative regulation of p38 mitogen-activated protein kinase. American journal of respiratory and critical care medicine, 183(8), 1015-1024.

Lyons, A., O'Mahony, D., O'Brien, F., MacSharry, J., Sheil, B., Ceddia, M., & O'Mahony, L. (2010). Bacterial strain‐specific induction of Foxp3+ T regulatory cells is protective in murine allergy models. Clinical & Experimental Allergy, 40(5), 811-819.

Wang, W., Zhu, Z., Zhu, B., & Ma, Z. (2011). Peroxisome Proliferator-Activated Receptor–γ Agonist Induces Regulatory T Cells in a Murine Model of Allergic Rhinitis. Otolaryngology--Head and Neck Surgery, 144(4), 506-513.

Breslow, R. G., Rao, J. J., Xing, W., Hong, D. I., Barrett, N. A., & Katz, H. R. (2010). Inhibition of Th2 adaptive immune responses and pulmonary inflammation by leukocyte Ig-like receptor B4 on dendritic cells. The journal of immunology, 184(2), 1003-1013.

Yadav, U. C., Naura, A. S., Aguilera-Aguirre, L., Ramana, K. V., Boldogh, I., Sur, S., & Srivastava, S. K. (2009). Aldose reductase inhibition suppresses the expression of Th2 cytokines and airway inflammation in ovalbumin-induced asthma in mice. The Journal of Immunology, 183(7), 4723-4732.

Park, S. J., Lee, K. S., Kim, S. R., Min, K. H., Choe, Y. H., Moon, H., ... & Lee, Y. C. (2009). Peroxisome proliferator-activated receptor γ agonist down-regulates IL-17 expression in a murine model of allergic airway inflammation. The Journal of Immunology, 183(5), 3259-3267.

Larsen, S. T., Roursgaard, M., Jensen, K. A., & Nielsen, G. D. (2010). Nano titanium dioxide particles promote allergic sensitization and lung inflammation in mice. Basic & clinical pharmacology & toxicology, 106(2), 114-117.

Lee, C. G., Hartl, D., Lee, G. R., Koller, B., Matsuura, H., Da Silva, C. A., ... & Elias, J. A. (2009). Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13–induced tissue responses and apoptosis. The Journal of experimental medicine, 206(5), 1149-1166.

Beigelman, A., Gunsten, S., Mikols, C. L., Vidavsky, I., Cannon, C. L., Brody, S. L., & Walter, M. J. (2009). Azithromycin attenuates airway inflammation in a noninfectious mouse model of allergic asthma. CHEST Journal, 136(2), 498-506.


Tissue-specific effects of allergic rhinitis in mouse nasal epithelia
Carr, V.M. et al., Chem Senses, Sep 2012; 37: 655 - 668. Lpa2 is a negative regulator of both dendritic cell activation and murine models of allergic lung inflammation
Emo, J et al., J. Immunol., Apr 2012; 188: 3784 - 3790Peroxisome Proliferator-Activated Receptor–γ Agonist Induces Regulatory T Cells in a Murine Model of Allergic Rhinitis
Wang, W. et al., Otolaryngology -- Head and Neck Surgery, Feb 2011A Cell-Impermeable Cyclosporine A Derivative Reduces Pathology in a Mouse Model of Allergic Lung Inflammation
Balsley, M et al., J. Immunol., Dec 2010; 185: 7663Inhibition of Th2 Adaptive Immune Responses and Pulmonary Inflammation by Leukocyte Ig-Like Receptor B4 on Dendritic Cells
Breslow, R et al., J. Immunol., Jan 2010; 184: 1003 - 1013.A Novel Dithiol Amide CB3 attenuates allergic airway disease through negative regulation of p38 MAPK
Kim SR, et al. Am. J. Respir. Crit. Care Med. April 2010Aldose Reductase Inhibition Suppresses the Expression of Th2 Cytokines and Airway Inflammation in Ovalbumin-Induced Asthma in Mice
Umesh C. S. Yadav et al., J. Immunol., Oct 2009; 183: 4723 - 4732.Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13–induced tissue responses and apoptosis
Chun Geun Lee et al., J. Exp. Med., May 2009; 206: 1149 - 1166.Peroxisome Proliferator-Activated Receptor Agonist Down-Regulates IL-17 Expression in a Murine Model of Allergic Airway Inflammation
Seoung Ju Park et al, J. Immunol., Sep 2009; 183: 3259 - 3267.Azithromycin Attenuates Airway Inflammation in a Noninfectious Mouse Model of Allergic Asthma
Avraham Beigelman et al., Chest, Aug 2009; 136: 498 - 506.

Product Insert (PDF) - Informational use only. Please refer to insert included with product.

Data/Specifications: 

  • Species: mouse
  • Sample Type : cell culture supernate, serum
  • Sample Preparation: dilute 1:10, adjust as needed
  • Test Volume: 100 uL
  • Length: 2 hours 15 min
  • Range: 7.8-500 ng/mL
  • Sensitivity: < 3.8 ng/mL