ST2L Human, Monoclonal Antibody, Biotinylated

Monoclonal Antibody to Human ST2L, Biotinylated, Clone B4E6
Catalog Number: 
101002B
Qty/Size: 
200 uL

Monoclonal Antibody to Human ST2L, Biotinylated, Clone B4E6 from MD Bioproducts

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Human ST2L biotinylated monoclonal antibody (Clone B4E6) for the identification and purification of human type 2 lymphocyte (Th2) subsets

Overview

Overview: 

T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for both murine and human Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.

Data/Specifications

Data/Specifications: 

Clone: B4E6

 

IgG Subclass: IgG1

 

Form: The biotinylated antibody is supplied in PBS and purified over protein G-sepharose from concentrated tissue culture supernatant. The antibody is supplied with 0.1% sodium azide as preservative.

 

Specificity: This clone recognizes hST2L specifically on the cell surface of human type 2 (Th2, Tc2, NK2 and NKT2) but not type 1 lymphocytes.

Literature/Support

Literature/Support: 

ST2L Monoclonal Antibody, Biotinylated (product insert)


Understanding The Four Isoforms of ST2 (blog post)

 

Gordon, E. D., Simpson, L. J., Rios, C. L., Ringel, L., Lachowicz-Scroggins, M. E., Peters, M. C., ... & Yuan, S. (2016). Alternative splicing of interleukin-33 and type 2 inflammation in asthma. Proceedings of the National Academy of Sciences113(31), 8765-8770.

References/Citations:The Antibody was used for:

Predominance of Th2 response in human abdominal aortic aneurysm: Mistaken identity for IL-4-producing NK and NKT cells?  Chan WL, et al. Cellular Immun (2005) 233:109-114

 

Application of ST2L and IL18R markers in absdominal aortic aneurysms
Changes in systemic type 1 and type 2 immunity in normal pregnancy and pre-eclampsia may be mediated by natural killer cells  Borzychowski, A.M. et al., Eur J Immunol (2005) 35:3054-3063. 

Demonstrates the use of ST2L and IL-18R in differentiating normal versus pre-eclamptic pregnancies.
Atherosclerotic Abdominal Aortic Aneurysm and the Interaction Between Autologous Human Plaque-Derived Vascular Smooth Muscle Cells, Type-1 NKT, and Helper T-Cells  Chan, W.L. et al., Circ Res (2005) 96:675-683

Use of Il-18R and ST2L in differentiating inflammatory cells in the presence of atherosclerotic plaques in Abdominal Aortic Aneurysm (AAA)
NKT cell subsets in infection and inflammation  Chan WL, et al. Immun Lett (2003) 85:159-163

Data demonstrating that ST2L and IL-18R could serve as important determinants of immune status in human diseases such as HIV, psoriasis, atherosclerosis

Regulation of ST2L expression on T helper (Th) type 2 cells  Carter, R.W. et al., Eur. J. Immunol. (2001) 31:2979-2985

Data providing mechanistic explanation for the selective expression of ST2L on Th2 cells
Human IL-18 Receptor and ST@L Are Stable and Selective markers for the Respective Type 1 and Type 2 Circulating Lymphocytes  Chan WL, et al. J Immunol. 2001 Aug 1; 167(3):1238-44.ST2L and IL-18R markers are stable cell surface markers serving as important determinants of the general immune status in human diseases, thereby useful as markers for therapeutic monitoring and intervention.



 

 

How To Use

How To Use: 

 

Applications

  • Flow cytometry
  • FACS
  • cell sorting
  • immunohistochemistry with hybridoma culture supernatant