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ST2L Human, Monoclonal Antibody, FITC

Monoclonal Antibody to Human ST2L, FITC Conjugated, Clone B4E6
Catalog Number: 
101002F
Qty/Size: 
200 uL

Monoclonal Antibody to Human ST2L, FITC Conjugated, Clone B4E6 from MD Bioproducts

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Human ST2L FITC conjugated monoclonal antibody (clone B4E6) for the identification and purification of human type 2 lymphocyte (Th2) subsets.

Overview

Overview: 

T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for both murine and human Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.

Data/Specifications

Data/Specifications: 

Clone: B4E6

 

IgG Subclass: IgG1

 

Form: The fluorescein (FITC) conjugated antibody is supplied in PBS and purified over protein Gsepharose from concentrated tissue culture supernatant. This antibody is supplied with 0.1% sodium azide as a preservative.

 

Specificity: This clone recognizes hST2L specifically on the cell surface of human type 2 (Th2, Tc2, NK2 and NKT2) but not type 1 lymphocytes.

Literature/Support

Literature/Support: 

Monoclonal Antibody to ST2L, FITC (product insert)

 

Understanding The Four Isoforms of ST2 (blog post)

 

Rönnberg, E., Ghaib, A., Ceriol, C., Enoksson, M., Arock, M., Säfholm, J., ... & Nilsson, G. (2018). Divergent effects of acute and prolonged interleukin 33 exposure on mast cell IgE-mediated functions. bioRxiv, 463950.

 

Wojno, E. T., Monticelli, L. A., Tran, S. V., Alenghat, T., Osborne, L. C., Thome, J. J., ... & Artis, D. (2015). The prostaglandin D 2 receptor CRTH2 regulates accumulation of group 2 innate lymphoid cells in the inflamed lung. Mucosal immunology8(6), 1313.

 

Hams, E., Armstrong, M. E., Barlow, J. L., Saunders, S. P., Schwartz, C., Cooke, G., ... & Voehringer, D. (2014). IL-25 and type 2 innate lymphoid cells induce pulmonary fibrosis. Proceedings of the National Academy of Sciences111(1), 367-372

 

Seltmann, J., Werfel, T., & Wittmann, M. (2013). Evidence for a regulatory loop between IFN‐γ and IL‐33 in skin inflammation. Experimental dermatology22(2), 102-107.

 

Hyde, R. K., Kamikubo, Y., Anderson, S., Kirby, M., Alemu, L., Zhao, L., & Liu, P. P. (2010). Cbfb/Runx1 repression–independent blockage of differentiation and accumulation of Csf2rb-expressing cells by Cbfb-MYH11. Blood115(7), 1433-1443.

 

van‘t Veer, C., van den Pangaart, P. S., van Zoelen, M. A., de Kruif, M., Birjmohun, R. S., Stroes, E. S., ... & van der Poll, T. (2007). Induction of IRAK-M is associated with lipopolysaccharide tolerance in a human endotoxemia model. The Journal of Immunology179(10), 7110-7120.

 

Abdulahad, W. H., van der Geld, Y. M., Stegeman, C. A., & Kallenberg, C. G. (2006). Persistent expansion of CD4+ effector memory T cells in Wegener's granulomatosis. Kidney international70(5), 938-947.

 

References/Citations:How the ST2L, FITC conjugated antibody was used:

IL-33 Induces Antigen-Specific IL-5+ T Cells and Promotes Allergic-Induced Airway Inflammation Independent of IL-4
Mariola Kurowska-Stolarska et al., J. Immunol., Oct 2008; 181: 4780 - 4790.

Flow cytometry analysis to assess ST2 expression profile in cultured or freshly isolated T cells from drained lymph nodes (DLN) of BALB/c wild type mice and from CD4+ T cells of D0.11.10 mice.

Induction of IRAK-M Is Associated with Lipopolysaccharide Tolerance in a Human Endotoxemia Model
Cornelis van ‘t Veer et al., J. Immunol., Nov 2007; 179: 7110 - 7120.

Flow cytometry analysis to assess the expression profile of ST2L in CD14high T cells from human whole blood collections.

The vast majority of CLA+ T cells are resident in normal skin.
Clark R, et al. J of immunology., 2006; 176(7): 4431-9.

Flow cytometry analysis to examine CD4+ skin resident T cells for Th1/Th2 polarization (ST2L molecule is limited to Th2 cells). T cells were isolated from normal adult human skin obtained from cutaneous surgeries. Work performed on BD Biosciences FACScan instrument, data analyzed using CellQuest software.

Predominance of Th2 response in human abdominal aortic aneurysm: Mistaken identity for IL-4-producing NK and NKT cells?  Chan WL, et al. Cellular Immun (2005) 233:109-114

 

Application of ST2L and IL18R markers in absdominal aortic aneurysms
Changes in systemic type 1 and type 2 immunity in normal pregnancy and pre-eclampsia may be mediated by natural killer cells  Borzychowski, A.M. et al., Eur J Immunol (2005) 35:3054-3063. 

Demonstrates the use of ST2L and IL-18R in differentiating normal versus pre-eclamptic pregnancies.
Atherosclerotic Abdominal Aortic Aneurysm and the Interaction Between Autologous Human Plaque-Derived Vascular Smooth Muscle Cells, Type-1 NKT, and Helper T-Cells  Chan, W.L. et al., Circ Res (2005) 96:675-683

Use of Il-18R and ST2L in differentiating inflammatory cells in the presence of atherosclerotic plaques in Abdominal Aortic Aneurysm (AAA)
NKT cell subsets in infection and inflammation  Chan WL, et al. Immun Lett (2003) 85:159-163

Data demonstrating that ST2L and IL-18R could serve as important determinants of immune status in human diseases such as HIV, psoriasis, atherosclerosis

Regulation of ST2L expression on T helper (Th) type 2 cells  Carter, R.W. et al., Eur. J. Immunol. (2001) 31:2979-2985

Data providing mechanistic explanation for the selective expression of ST2L on Th2 cells
Human IL-18 Receptor and ST@L Are Stable and Selective markers for the Respective Type 1 and Type 2 Circulating Lymphocytes  Chan WL, et al. J Immunol. 2001 Aug 1; 167(3):1238-44.ST2L and IL-18R markers are stable cell surface markers serving as important determinants of the general immune status in human diseases, thereby useful as markers for therapeutic monitoring and intervention.

 

 

 

How To Use

How To Use: 

 

Applications

  • Flow cytometry
  • FACS
  • cell sorting
  • immunohistochemistry with hybridoma culture supernatant