ArthritoMab™ Antibody Cocktail

Catalog Number: 
CIA-MAB-50
Qty/Size: 
50 mg

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ArthritoMab™ Antibody Cocktail is a cocktail of 4 monoclonal antibodies to collagen II (CII) used for inducing arthritis in the anti-Collagen Antibody Induced Arthritis (CAIA) model.

Overview

Overview: 

Inducing severe arthritis in mice typically within 24-48 hrs using a cocktail of monoclonal antibodies selected for their epitope specificity.

Collagen-induced arthritis (CIA) in mice is widely used as an experimental model for rheumatoid arthritis (RA) in humans. CIA is mediated by autoantibodies, which bind to a particular region of type II collagen (CII). The ability to induce arthritis using this arthritogenic antibody cocktail provides an efficient protocol for the induction of antibody-mediated arthritis that can be used as a shorter, more synchronized alternative to the CIA model.

Benefits of the CAIA model

  • Length of study: Arthritis develops in mice typically within 24-48 hr allowing the completion of a study within 2 weeks reducing the number of assessments and scoring periods.
  • Reduced group size: Rate of incidence is nearly 100% depending on the strain allowing for smaller group sizes.
  • Synchronization: onset of disease is synchronized between animals simplifying treatment schedules.
  • Susceptibility: Arthritis is induced not only in CIA-susceptible DBA/1 and B10.RIII mice, but also in some CIA-resistant mice, such as Balb/c.

CIA vs CAIA

Abreviations:

  • Anti-Collagen Induced arthritis (ACIA)
  • Monoclonal Antibody induced arthritis (mAb-RA)
  • Collagen Antibody Induced Arthritis (CAIA)

Data/Specifications

Data/Specifications: 


Epitope specificity

This arthritogenic cocktail of 4 monoclonal antibodies bind to the well-defined epitopes C11b, J1, D3 and U1, which are spread over the entire CII (CB8, CB10 and CB11 fragments), possibly encouraging better immune complex formation on the cartilage surface for the initiation of arthritis.

Clones

Comprised of 4 antibodies to Collagen II:

  • clone M2139 (IgG2b)
  • clone CIIC2 (IgG2a)
  • clone UL1 (IgG2b)
  • clone CIIC1 (IgG2b)

Susceptibility 

  • Balb/c
  • DBA/1
  • B10.RIII
  • C57Bl/6
Histology various strainsClinical scores of various strains

Literature/Support

Literature/Support: 

 

References/Citations

Mast cell chymase contributes to the antibody response and the severity of autoimmune arthritis

Sofia E. Magnusson, Gunnar Pejler, Sandra Kleinau, and Magnus Åbrink FASEB J, Mar 2009; 23: 875 - 882.

 

The novel small molecule drug Rabeximod is effective in reducing disease severity of mouse models of autoimmune disorders

M Hultqvist, K S Nandakumar, U Björklund, and R Holmdahl Ann Rheum Dis, Jan 2009; 68: 130 - 135.

 

Dominant-Negative Inhibitors of Soluble TNF Attenuate Experimental Arthritis without Suppressing Innate Immunity to Infection

Jonathan Zalevsky et al., J. Immunol., Aug 2007; 179: 1872 - 1883.

 

PRECLINICAL EFFICACY OF XPROTM 1595, A BIOLOGIC DOMINANT-NEGATIVE INHIBITOR OF SOLUBLE TNF THAT BLOCKS INFLAMMATION WITHOUT SUPPRESSING INNATE IMMUNITY

J. Zalevsky, P. Wong, C. O'Brien, and D.E. Szymkowski Ann Rheum Dis, Jun 2006; 65: 142.

 

 

How To Use

How To Use: 

Applications

 

  • In vivo: induce disease in the collagen antibody induced arthritis model
  • bind collagen in vivo
  • impair cartilage formation by chondrocytes in vitro
  • inhibit assembly of CII in vitro
  • inhibit collagen fibrillogenesis in vitro
For the Induction of Arthritis in the Collagen Antibody Induced Arthritis Model:
  1. Administer antibody cocktail on day 0*
  2. Boost with LPS on day 3
  3. Terminate on day 12
*Amount of cocktail will vary depending on animal source and strain, housing conditions, feed and water. Each lab must optimize for their own conditions.