Endorphin (β-Endorphin) ELISA

The β-endorphin ELISA kit is designed to measure β-Endorphin levels in human sam
Catalog Number: 
M056011
Qty/Size: 
96 wells

beta-endorphin ELISA from MD Bioproducts

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The β-endorphin ELISA kit is designed to measure β-Endorphin levels in human samples.

Overview

Overview: 

β-endorphin is an endogenous opioid peptide neurotransmitter found in the neurons of both the central and peripheral nervous system. It is a 31 amino acids long resulting from processing of the precursor proopiomelanocortin (POMC), which also gives rise to the other peptide hormones including ACTH (adrenocorticotropic hormone) and α- and γ-MSH (melanocyte-stimulating hormone). β-endorphin is an agonist of opioid receptors giving it analgesic properties. 

 

 

Data/Specifications

Data/Specifications: 

Species: human

Sample Type:  serum, plasma

Sample Preparation: plasma extraction

Sample Size: 50 uL

Standard Curve Range:  0.01 - 100 ng/mL

Sensitivity:  1 ng/mL

Assay Length:   4 hrs

Cross-reactivity:

Leu-Enkephalin: 0 %

MET-Enkephalin: 0 %

beta_Endorphin (Human): 100 %

alpha Endorphin: 0 %

Gamma Endorphin: 0 %

Literature/Support

Literature/Support: 

Product Insert:

β-endorphin ELISA Insert (PDF)

 

Articles/Troublshooting:

ELISA Troubleshooting Guide

ELISA Data Reduction Guide

 

Viet, C. T., Dang, D., Ye, Y., Ono, K., Campbell, R. R., & Schmidt, B. L. (2014). Demethylating drugs as novel analgesics for cancer pain. Clinical Cancer Research, 20(18), 4882-4893.

 

Milman, S., Leu, J., Shamoon, H., Vele, S., & Gabriely, I. (2012). Opioid receptor blockade prevents exercise-associated autonomic failure in humans. Diabetes, 61(6), 1609-1615.

 

Milman, S., Leu, J., Shamoon, H., Vele, S., & Gabriely, I. (2011). Magnitude of exercise-induced β-endorphin response is associated with subsequent development of altered hypoglycemia counterregulation. The Journal of Clinical Endocrinology & Metabolism, 97(2), 623-631.

 

Vele, S., Milman, S., Shamoon, H., & Gabriely, I. (2011). Opioid receptor blockade improves hypoglycemia-associated autonomic failure in type 1 diabetes mellitus. The Journal of Clinical Endocrinology & Metabolism, 96(11), 3424-3431.

 

Viet, C. T., Ye, Y., Dang, D., Lam, D. K., Achdjian, S., Zhang, J., & Schmidt, B. L. (2011). Re-expression of the methylated EDNRB gene in oral squamous cell carcinoma attenuates cancer-induced pain. PAIN®, 152(10), 2323-2332. 

 

References/Citations:The Beta-Endorphin ELISA Kit was used for:
Validation of a sham acupuncture procedure in a randomised, controlled clinical trial of chronic pelvic pain treatment
Lee, SW et al., Acupunct Med. 2011
Measure the concentration of beta-endorphin in accupuncture treated patient group.
Vernonia cinerea Less. supplementation and strenuous exercise reduce smoking rate: relation to oxidative stress status and beta-endorphin release in active smokers.
Leelarungrayub D, et al. J Int Soc Sports Nutr. 2010 May; 7:21.
Measure the concentration of beta-endorphin in human plasma samples.
Propofol/alfentanil and propofol/ketamine procedural sedation in children with acute lymphoblastic leukaemia: safety, efficacy and their correlation with pain neuromediator expression.
Chiaretti A, et al. Eur J Cancer Care. 2010 Mar; 19(2):212-20.
 

Endothelin-A Receptor Antagonism Attenuates Carcinoma-Induced Pain Through Opioids in Mice.
Quang PN and Schmidt BL. J Pain. 2010 Jan (Epub ahead of print)

Measure the concentration of BE in media collected from squamous cell carcinoma (SCC) cell culture. The SCC cell line was derived from human tongue.
The Effects of Hydrotherapy on Anxiety, Pain, Neuroendocrine Responses, and Contraction Dynamics During Labor.
Benfield RD, et al. Biol Res Nurs. 2010 May
Measure the concentration of BE in plasma samples from female humans. Plasma was prepared from whole blood collected in vacutainers containing EDTA. Traysylol (5,000 KIU) was added to inhibit proteolysis. Blood was spun at 3,000 rpm for 15 minutes at 14 °C and the plasma was collected, stored at -80 °C.
Biologically active breast milk proteins in association with very preterm delivery and stage of lactation.
Mehta R and Petrova A. J Perinatol. 2010 Jun
Measure the concentration of beta-endorphin in breast milk samples obtained from female humans.
Serum beta-endorphin response to stress before and after operation under fentanyl anesthesia in neonates, infants and preschool children.
Mirilas P, et al. Eur J Pediatr Surg. 2010 Mar; 20(2):106-10.
Measure concentration of serum BE in human samples.
Clinical and endocrinological changes after electro-acupuncture treatment in patients with osteoarthritis of the knee.
Ahsin S, et al. Pain. 2009 Dec; 147(1-3):60-6.
Measure the concentration of beta-endorphin in plasma from human patients with osteoarthritis of the knee. Plasma was obtained from blood collected in a pre-cooled EDTA tube.
Peripheral endothelin B receptor agonist-induced antinociception involves endogenous opioids in mice.
Quang PN and Schmidt BL. Pain. 2010 May; 149(2):254-62.
Measure the concentration of BE in media collected from squamous cell carcinoma (SCC) cell culture. The SCC cell line was derived from human tongue.
Beta-endorphin in serum and seminal plasma in infertile men.
El-Hagger S, et al. Asian J Androl. 2006 Jun; 8(6):709-12.
Measure the concentration of beta-endorphin in seminal plasma and serum from male human patients.
Cognitive and emotional factors in placebo analgesia.
Flaten MA, et al. J Psychosom Res. 2006 Jul; 61(1):81-9.
Measure the concentration of serum BE from adult humans. Serum was prepared from whole blood by centrifugation at 1500g for 10 minutes.

Plasma beta-endorphin levels in frequent and infrequent tanners before and after ultraviolet and non-ultraviolet stimuli.
Kaur M, et al. J Am Acad Dermatol. 2006 May;54(5):919-20.

Measure the concentration of beta-endorphin in plasma from female human subjects exposed to Ultraviolet light.
Treatment with double dose of omezprazole increases Beta Endorphin plasma in patients with coronary artery disease.
Budzynski J, et al. Archives of Medical Science. 2010; 6(2):201-207.
 

 

 

How It Works

How It Works: 

The immunoplate in this kit is pre-coated with secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in sample. The biotinylated peptide is able to interact with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate solution composed of 3,3’,5,5’-tetramethylbenzidine (TMB) and hydrogen peroxide to produce a blue colored solution. The enzyme-substrate reaction is stopped by hydrogen chloride (HCl) and the solution turns to yellow. The intensity of the yellow is directly proportional to the amount of biotinylated peptide-SA/HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide and the peptide in standard solutions or samples to the peptide antibody (primary antibody). A standard curve of a peptide with known concentration can be established accordingly. The peptide with unknown concentration in samples can be determined by extrapolation to this standard curve.

 

beta-endorphin ELISA

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