The PTH ELISA kit is intended to measure Intact PTH levels in human serum and plasma samples. Assay time is 3. 5 hours.    PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized...



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For research use only. Not for use in diagnostic procedures.

The PTH ELISA kit is intended to measure Intact PTH levels in human serum and plasma samples. Assay time is 3. 5 hours. 


PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a pre-proparathyroid hormone, a larger molecular precursor consisting of 115 amino acids. Following sequential intracellular cleavage of a 25-amino acid sequence, preproparathyroid hormone is converted to an intermediate, a 90-amino acid polypetide, proparathyroid hormone. With additional proteolytic modification, proparathyroid hormone is then converted to parathyroid hormone, an 84 amino acid polypeptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and midregion fragments. In subjects with renal insufficiency, C-terminal and midregion PTH assays typically give elevated PTH results, as reflected by impaired renal clearance.


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    Cholecalciferol Supplementation throughout Winter Does Not Affect Markers of Bone Turnover in Healthy Young and Elderly Adults
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    Product Insert (PDF) - Informational use only. Please refer to insert included with product.


    • Species: human
    • Sample Type: serum, plasma
    • Sample Size: 25uL
    • Standard Curve Range: 7 - 700 pg/mL
    • Sensitivity: 0.9 pg/mL
    • Assay Length: 3.5 hrs