Collagen-induced arthritis (CIA) Reagents and Induction Method
The Collagen Induced Arthritis (CIA) model is a commonly used model as it shares immunological and pathological similarities to human RA. Arthritis is initiated by intradermal injections of Collagen Type II (CII) emulsified in Complete Freund's Adjuvant (CFA). This causes an immune response generating antibodies to CII. Therefore there is both a T cell and B cell component to the pathology. The joint destruction in collagen-induced arthritis involves cartilage destruction, bone resorption, synovial hyperplasia and periarticular cell infiltration thereby sharing many similarities to human RA.
As researchers rely on preclinical modeling of disease to evaluate potential therapeutics, it is crucial that successful induction and variability within and between studies is reduced to provide reliable data.
MD Bioproducts has over 25 years experiencing running the Collagen-induced arthritis model. Here we discuss common issues related to the induction of Collagen-induced arthritis in animal models of disease.
Mouse Strain and housing conditions:
- Male DBA/1 mice or C57Bl/6 mice are recommended.
- Age of animals: 7-8 weeks old
- House in SPF conditions and feed high fat diet.
Reagents for Emulsion
- Use only highly purified collagen type II (Bovine collagen type II or chicken collagen type II) at 2 mg/ml.
- Complete Freund's Adjuvant at 4 mg/ml
- Incomplete freund's adjuvant may be used as a booster if needed in DBA animals.