Mar 11 , 2021
March 11 is World Kidney Day. In honor of that, we are highlighting the Uromodulin (Tamm-Horsfall Protein) ELISA.
Our highly cited Uromodulin (Tamm-Horsfall Protein) ELISA was optimized during its development to provide precise and reproducible results. Selection of antibody pairs, careful control of microplate coating, formulation of reagents and protocol design along with stringent quality control procedures compile a complete ELISA kit that provides researchers a rapid and easy to use assay.
Tamm-Horsfall protein (THP, also known as uromodulin) is the most abundant protein found in the urine of mammals. THP is 616 amino acids in length with a molecular weight of 80 kDa, and is synthesized in the thick ascending loop of Henle. While the specific function of THP is unknown, it appears to have a role in the regulation of salt and water excretion by the kidney. Soluble THP has been found to help protect against urinary tract infections of E. coli and P. mirabilis by inhibiting the binding of the bacteria to the epithelial cells of the urinary tract. In addition, it has been suggested that THP may help prevent renal stone formation. Defects in THP are associated with human diseases such as familial juvenile hyperuricemic nephropathy (FJHN) and medullary cystic kidney disease (MCKD2).
Selection of Citations:
Steubl, D., Buzkova, P., Ix, J. H., Devarajan, P., Bennett, M. R., Chaves, P. H., ... & Garimella, P. S. (2020). Association of serum and urinary uromodulin and their correlates in older adults—The Cardiovascular Health Study. Nephrology, 25(7), 522-526.
Amatruda, J. G., Katz, R., Peralta, C. A., Estrella, M. M., Sarathy, H., Fried, L. F., & Health ABC Study. (2020). Association of Non‐Steroidal Anti‐Inflammatory Drugs with Kidney Health in Ambulatory Older Adults. Journal of the American Geriatrics Society.
Garimella, P. S., Jaber, B. L., Tighiouart, H., Liangos, O., Bennett, M. R., Devarajan, P., & Sarnak, M. J. (2017). Association of preoperative urinary uromodulin with AKI after cardiac surgery. Clinical Journal of the American Society of Nephrology, 12(1), 10-18.
Tomlinson, J. A., Caplin, B., Boruc, O., Bruce-Cobbold, C., Cutillas, P., Dormann, D., ... & Leiper, J. (2015). Reduced renal methylarginine metabolism protects against progressive kidney damage. Journal of the American Society of Nephrology, 26(12), 3045-3059.