Featured Publication in Focus: Gut–Joint Axis: Impact of Bifidobacterial Cell Wall Lipoproteins on Arthritis Development

Featured Publication in Focus: Gut–Joint Axis: Impact of Bifidobacterial Cell Wall Lipoproteins on Arthritis Development

Jan 29 , 2024

MD Bioproducts

Authors:

Frank Piva, Philippe Gervois, Youness Karrout, Famara Sané and Marie-Bndicte Romond

 

Virology Laboratory-ULR3610, University of Lille and CHU Lille, 59000 Lille, France

Inserm U1008, University of Lille and CHU Lille, 59000 Lille, France

 

MDPI. Nutrients. 2023 November 21.

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Products referenced:

Catalogue # CIA-MAB-50

ArthritoMab™ Antibody Cocktail for Balb/c, DBA/1, R10.RIII, 50 mg

Catalogue # 804001-lyo

Collagen Type II, Bovine, Immunization Grade, Lyophilized, 10mg

 

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ABSTRACT

Gut microbiota affect progression of rheumatoid arthritis (RA). The present study aims at investigating the protective potential of Bifidobacterium longum cell wall lipoproteins (Lpps) shown to modulate the intestinal microbiome and prevent osteoarthritis. Arthritis was induced by collagen (CIA) or anti-collagen antibodies (CAIA) injection. Intake of 0.5 mg of Lpps/L, but not 0.25 and 1 mg of Lpps/L, significantly alleviated RA symptoms in CIA DBA/1OOaHsd mice. The arthritis index (AI) was also reduced in CAIA mice. In the CIA-protected group, colon Ligilactobacillus murinus, caecal Lactobacillus johnsonii and spleen weight correlated with AI, whereas the reverse was observed with splenic CD11c+ dendritic cells (cDCs). The unprotected CIA Lpps group harbored higher cecal and colon E. coli and lower caecal L. murinus. Lpps administration to CAIA mice after arthritis induction led to lower colon E. plexicaudatum counts. Splenocytes from CIA-protected mice triggered by LPS secreted higher Il-10 than control ones. However, a higher IL-10 response was not elicited in gnotobiotic RA mice splenocytes with lower cDCs’ recruitment. Labeled bacteria with the Lpps signal were detected in CIA mice bone marrow (BM) cDCs 5 and 16 h post-gavage but not in Peyer’s patches and the spleen. In vitro uptake of Lpps by primary BM and thymus cells was observed within 24 h. An FACS analysis detected the Lpps signal in the plasmacytoid cell compartment but not in cDCs. In conclusion, Lpps dosing is critical for preventing arthritis progression and appropriately modulating the microbiome. Our results also highlight the possible triggering of the immune system by Lpps.

 

To continue reading and to download the publication:

https://doi.org/10.3390/nu15234861