PCR Mycoplasma Detection, 20 assays
The PCR Mycoplasma test kit provides a rapid and sensitive PCR method for monitoring your cell cultures for mycoplasma contamination. Infections usually result in mycoplasma titers of 105 –108 CFU/mL Cell Cultures...
Reagent
409010
The PCR Mycoplasma test kit provides a rapid and sensitive PCR method for monitoring your cell cultures for mycoplasma contamination. Infections usually result in mycoplasma titers of 105 –108 CFU/mL
Cell Cultures are used every day as a part of research methods. You rely on these cultures to be healthy, yet up to 35% may be contaminated due to mycoplasma. Mycoplasma contamination affects many cell functions including metabolism, morphology, protein synthesis and cell proliferation - all of which lead to unreliable results as well as lost time and resources. Since contamination is not typically visible by turbidity, routine screening for mycoplasma contamination is essential.
References/Citations:
Campbell, B. B., Galati, M. A., Stone, S. C., Riemenschneider, A. N., Edwards, M., Sudhaman, S., ... & Tabori, U. (2021). Mutations in the RAS/MAPK Pathway Drive Replication Repair–Deficient Hypermutated Tumors and Confer Sensitivity to MEK Inhibition. Cancer discovery, 11(6), 1454-1467.
Wu, Y., Yang, Z., Denslin, V., Ren, X., Lee, C. S., Yap, F. L., & Lee, E. H. (2020). Repair of Osteochondral Defects With Predifferentiated Mesenchymal Stem Cells of Distinct Phenotypic Character Derived From a Nanotopographic Platform. The American Journal of Sports Medicine, 0363546520907137.
Parreno, J., Bianchi, V. J., Sermer, C., Regmi, S. C., Backstein, D., Schmidt, T. A., & Kandel, R. A. (2018). Adherent agarose mold cultures: An in vitro platform for multifactorial assessment of passaged chondrocyte redifferentiation. Journal of Orthopaedic Research®, 36(9), 2392-2405.
Warnecke, D., Schild, N. B., Klose, S., Joos, H., Brenner, R. E., Kessler, O., ... & Dürselen, L. (2017). Friction properties of a new silk fibroin scaffold for meniscal replacement. Tribology international, 109, 586-592.
Pallante-Kichura, A. L., Chen, A. C., Temple-Wong, M. M., Bugbee, W. D., & Sah, R. L. (2013). In vivo efficacy of fresh versus frozen osteochondral allografts in the goat at 6 months is associated with PRG4 secretion. Journal of Orthopaedic Research, 31(6), 880-886.
Product Insert (PDF) - Informational use only. Please refer to insert included with product.
Data/Specifications:
The PCR Mycoplasma Test Kit is adequate to diagnose cell cultures infected with mycoplasmas. Infections usually result in mycoplasma titers of 105 –108 CFU/mL
The kit allows detection of Acholeplasma and Spiroplasma and the following Mycoplasma species:
- M. fermentans
- M. hyorhinis
- M. arginini
- M. orale
- M. salivarium
- M. hominis
- M. pulmonis
- M. arthritidis
- M. bovis
- M. pneumoniae
- M. pirum
- M. capricolum
Sensitivity
Type of Mycoplasma | Minimum Concentration Detected |
M. FERMENTANS | 240 CFU/mL |
M. CAPRICOLOM | 110 CFU/mL |
PENETRANS | 200 CFU/mL |
HYORHINIS | 210 CRU/mL |