MonELISA® Canine IL-17a is an assay using antibodies generated specifically to the entire canine IL-17a protein without any cross-reactivity to other species. This assay provides the most specific results, combined with ease of use and the uniform MonELISA® assay procedure.

Requires MonELISA® Ancillary Reagent Kit, Catalog Number M036080 (sold separately). 

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The IL-17 family of cytokines contains six known members as part of the immune system. IL-17a and the structurally close IL-17f play a central role in immune response to pathogens and the development of various inflammatory autoimmune diseases. Psoriasis is one of the most-studied diseases featuring a central role for IL-17a. 

This species-specific MonELISA® IL-17a ELISA is a colorimetric based sandwich immunoassay utilizing a polyclonal antibody to the specie’s IL-17a bound on the surface of microwells as the capture antibody and a biotinylated polyclonal antibody to IL-17a as the detection antibody. When antigen is added to the well it is bound by the immobilized antibody. The biotinylated antibody then binds to the antigen forming an antigen-antibody complex. Streptavidin-Peroxidase in the presence of an enzyme substrate quantifies analyte bound.  Color development is directly proportional to the antigen concentration in the sample.