ACC4 (IgG1), Monoclonal Antibody, 100ug

This antibody is an anti-citrullinated protein antibody (ACPA) that, on Collagen alpha-1 (II) chain, binds specifically to citrullinated C1 epitope on alpha-chain (position 359–369). It cross-reacts with mouse, bovine, and potentially...

Product Type : Antibodies

SKU: 1061004

Citrullination of proteins is a post-translational modification in which arginine is converted into citrulline by a family of enzymes called peptidylarginine deiminases (PADs). Proteins are susceptible to citrullination during inflammatory processes, and an autoantibody response to citrullinated proteins is a hallmark of Rheumatoid Arthritis.

Synonyms:
COL2A1, Collagen Type II, Collagen alpha-1(II) chain, CII, Col II, citrullinated CII; citrullinated Col II; citrullinated proteins.

Monoclonal Antibody to ACC4 (IgG1) Citrullinated Collagen Type II, Affinity Purified

The mouse Anti-citrullinated COL2A1 Antibody clone ACC4 binds to the C1 epitope on alpha-chain of collagen II when the first arginine in the epitope (position 359–369; is citrullinated (Cit- Arg). The antibody does not bind to the C1 epitope when the second arginine is citrullinated (Arg-Cit), neither when both arginines are citrullinated (Cit- Cit). The antibody binds to the epitope in both triple helical and cyclic form. Binding to collagen II epitopes was determined in bead-based flow immunoassay as median fluorescence intensity (MFI).

References:

1. He, Y., Ge, C., Moreno-Giró, À. et al. A subset of antibodies targeting citrullinated proteins confers protection from rheumatoid arthritis. Nat Commun 14, 691 (2023).

2. Uysal H, Bockermann R, Nandakumar KS, Sehnert B, Bajtner E, Engström A, Serre G, Burkhardt H, Thunnissen MM, Holmdahl R. Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis. J Exp Med. 2009 Feb 16;206(2):449-62.

Product Insert (PDF) - Informational use only. Please refer to insert included with product.

Clone: ACC4
Host/Isotype: Mouse IgG1,k
Immunogen: Generated by immunization of DBA/1 or (B10.Q × DBA/1) F1 mice with Protein- arginine deiminase type-4 (PAD4) -treated triple-helical CII peptides containing T and B cell epitopes.
Species Cross-Reactivity: Mouse, Bovine, potentially Human
Form: This antibody is soluble and supplied in PBS.
Application: Immunohistochemistry (IHC), in-vivo functional studies such Collagen- Induced Arthritis (CIA) , and evoked pain-behavior
Suggested Dilution: It is recommended that the user determine the optimal dilution for their application. The typical starting working dilutions are for IHC 1:100.

ACC4 (IgG1), Monoclonal Antibody, 100ug

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