ACPA, Monoclonal Antibody (Clone E4NG), 100ug

Product Type : Antibodies

SKU: 1061006

This antibody recognizes multiple citrullinated proteins/peptides, including CCP2, citrullinated collagen type 2 (COL2) peptides, citrullinated human/mouse alpha-enolase (citENO1), etc., and potentially other citrullinated proteins/peptides typically manifested in rheumatoid arthritis, as a citrulline-specific antibody. E4NG has a mutation on glycosylation sites in the variable domain, prohibiting the expression of the Fab-glycan (no N-glycosylation on the variable domain). It cross-reacts with mouse and human.

Note: For negative control antibody use Catalogue number 1061006-NC

Anti-citrullinated protein antibodies (ACPA) are autoantibodies linked to rheumatoid arthritis (RA). In RA research, collagen-induced arthritis (CIA) or CAIA mouse models are used to study ACPA's role in disease mechanisms like inflammation and joint damage.

Synonyms:
Citrullinated epitopes of COL2, Collagen alpha-1(II) chain, COL2A1, type II collagen, CII1; Citrullinated epitopes of ENO1, MPB

Monoclonal Antibody to ACPA Anti–Citrullinated Protein Antibody, Affinity Purified

The antibody clone E4NG (Catalogue number 1061006) binds to citrullinated collagen type II (COL2) epitopes. E4NG-mutant (Catalogue number 1061006-NC) is the control antibody that does not bind to citrulline. Binding to unmodified COL2 peptides is low by both E4NG and E4NG-mutant antibodies. A multiplex flow immunoassay determined binding. (Results published in He et al Nat Comm 2023)

References:

1. He, Y., Ge, C., Moreno-Giró, À. et al. A subset of antibodies targeting citrullinated proteins confers protection from rheumatoid arthritis. Nat Commun 14, 691 (2023).

Product Insert (PDF) - Informational use only. Please refer to insert included with product.

Data/Specifications:

Clone: E4NG
Host/Isotype: Mouse IgG2b, k
Immunogen: Initially derived from a rheumatoid arthritis (RA) patient, selected by a single B cell isolated from an RA patient with high reactivity to cyclic citrullinated peptide 2 (CCP2). The antibody is recombinantly expressed in HEK cells as a chimeric antibody (human variable domain + mouse constant domain).
Species Cross-Reactivity: Mouse, Human
Form: This antibody is soluble and supplied in PBS.
Application: Immunohistochemistry IHC), Immunoprecipitation, ELISA, Multiplex Immunoassay, etc., is an ideal antibody for detecting citrullinated antigens. Additionally, in vivo and in vitro functional analysis also demonstrated that E4NG ACPA could protect against collagen antibody-induced arthritis (CAIA) in mice. Note: Negative control antibody: Catalogue number 1061006-NC
Suggested Dilution: It is recommended that the user determine the optimal dilution for their application. The typical starting working dilutions are for IHC 1:100-1:500, ELISA 1:500-1:1000.

ACPA, Monoclonal Antibody (Clone E4NG), 100ug

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