Collagen Type II, Monoclonal Antibody (Clone CIIC1), 100ug

This antibody binds specifically to the C1 epitope on triple helica collagen type 2. It cross-reacts with mouse, rat, chicken, baboon and horse. Collagen type II, encoded by the COL2A1 gene,...

Product Type : Antibodies

SKU: 1061009

This antibody binds specifically to the C1 epitope on triple helica collagen type 2. It cross-reacts with mouse, rat, chicken, baboon and horse.

Collagen type II, encoded by the COL2A1 gene, is a crucial component of cartilage and plays a vital role in maintaining joint integrity. In mouse models of arthritis, such as collagen-induced arthritis, COL2A1 is studied for its role in cartilage degradation, a key feature of conditions like rheumatoid arthritis and osteoarthritis. Research on COL2A1 helps in understanding arthritis mechanisms and developing new treatments.

Synonyms:
Collagen alpha-1(II) chain, COL2A1, Collagen Type II, Collagen Type 2, CII.

Monoclonal Antibody to Collagen Type II (Clone CIIC1) (COL2A1), Affinity Purified

Anti-COL2A1 Antibody (CIIC1) binds to the triple helical C1 epitope on collagen type 2 (CII) but not when the epitope is double-citrullinated (Cit-Cit). CIIC1 can be used in studies of collagen citrullination together with mouse Anti-citrullinated COL2A1 ACC4 Antibody (Catalogue number 1061004). Binding specificity to triple helical C1 peptide epitopes was determined by bead-based flow immunoassay as median fluorescence intensity (MFI).

References:

1. Holmdahl, R., et al. Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti- type II collagen antibodies. Arthritis Rheum. 1986. 29: 400–410.

2. Nandakumar KS, et al. Collagen type II-specific monoclonal antibody- induced arthritis in mice: description of the disease and the influence of age, sex, and genes. Am J Pathol. 2003 Nov;163(5):1827-37.

3. Nandakumar KS, et al. Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells. Arthritis Res Ther. 2004;6(6):R544-50.

4. Uysal H, et al. The crystal structure of the pathogenic collagen type II- specific mouse monoclonal antibody CIIC1 Fab: structure to function analysis. Mol Immunol. 2008 Apr;45(8):2196-204.

5. Böiers U, et al. Collagen type II is recognized by a pathogenic antibody through germline encoded structures. Eur J Immunol. 2008 Oct;38(10):2784-95.

Product Insert (PDF) - Informational use only. Please refer to insert included with product.

Data/Specifications:

Clone: CIIC1
Host/Isotype: Human IgG1,k
Immunogen: Derived from B cells of collagen type 2 immunized DBA/1 mice.
Species Cross-Reactivity: Mouse, Rat, Chicken, Horse, Baboon
Form: This antibody is soluble and supplied in PBS.
Application: Immunohistochemistry (IHC), ELISA, in vivo functional assays, such as Collagen-Induced Arthritis.
Suggested Dilution: It is recommended that the user determine the optimal dilution for their application. The typical starting working dilutions are IHC 1:100 and ELISA 1:1000.

Collagen Type II, Monoclonal Antibody (Clone CIIC1), 100ug

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