PCR Mycoplasma Detection Kit, 20 assays

The PCR Mycoplasma test kit provides a rapid and sensitive PCR method for monitoring your cell cultures for mycoplasma contamination.  Infections usually result in mycoplasma titers of 105 –108 CFU/mL Cell Cultures...

Reagent

409010

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For research use only. Not for use in diagnostic procedures.

The PCR Mycoplasma test kit provides a rapid and sensitive PCR method for monitoring your cell cultures for mycoplasma contamination.  Infections usually result in mycoplasma titers of 105 –108 CFU/mL

Cell Cultures are used every day as a part of research methods. You rely on these cultures to be healthy, yet up to 35% may be contaminated due to mycoplasma. Mycoplasma contamination affects many cell functions including metabolism, morphology, protein synthesis and cell proliferation - all of which lead to unreliable results as well as lost time and resources. Since contamination is not typically visible by turbidity, routine screening for mycoplasma contamination is essential.


References/Citations:

Hariharan, A., Qi, W., Rehrauer, H., Wu, L., Ronner, M., Wipplinger, M., ... & Felley‐Bosco, E. (2022). Heterogeneous RNA editing and influence of ADAR2 on mesothelioma chemoresistance and the tumor microenvironment. Molecular Oncology, 16(22), 3949.

Campbell, B. B., Galati, M. A., Stone, S. C., Riemenschneider, A. N., Edwards, M., Sudhaman, S., ... & Tabori, U. (2021). Mutations in the RAS/MAPK Pathway Drive Replication Repair–Deficient Hypermutated Tumors and Confer Sensitivity to MEK Inhibition. Cancer discovery11(6), 1454-1467.

Wu, Y., Yang, Z., Denslin, V., Ren, X., Lee, C. S., Yap, F. L., & Lee, E. H. (2020). Repair of Osteochondral Defects With Predifferentiated Mesenchymal Stem Cells of Distinct Phenotypic Character Derived From a Nanotopographic Platform. The American Journal of Sports Medicine, 0363546520907137.

Okonska, A., Bühler, S., Rao, V., Ronner, M., Blijlevens, M., van der Meulen-Muileman, I. H., ... & Felley-Bosco, E. (2020). Functional genomic screen in mesothelioma reveals that loss of function of BRCA1-associated protein 1 induces chemoresistance to ribonucleotide reductase inhibition. Molecular Cancer Therapeutics, 19(2), 552-563.

Parreno, J., Bianchi, V. J., Sermer, C., Regmi, S. C., Backstein, D., Schmidt, T. A., & Kandel, R. A. (2018). Adherent agarose mold cultures: An in vitro platform for multifactorial assessment of passaged chondrocyte redifferentiation. Journal of Orthopaedic Research®, 36(9), 2392-2405.

Warnecke, D., Schild, N. B., Klose, S., Joos, H., Brenner, R. E., Kessler, O., ... & Dürselen, L. (2017). Friction properties of a new silk fibroin scaffold for meniscal replacement. Tribology international, 109, 586-592.

Pallante-Kichura, A. L., Chen, A. C., Temple-Wong, M. M., Bugbee, W. D., & Sah, R. L. (2013). In vivo efficacy of fresh versus frozen osteochondral allografts in the goat at 6 months is associated with PRG4 secretion. Journal of Orthopaedic Research, 31(6), 880-886.

Product Insert (PDF) - Informational use only. Please refer to insert included with product.

Data/Specifications: 

The PCR Mycoplasma Test Kit is adequate to diagnose cell cultures infected with mycoplasmas. Infections usually result in mycoplasma titers of 105 –108 CFU/mL

The kit allows detection of Acholeplasma and Spiroplasma and the following Mycoplasma species:

  • M. fermentans
  • M. hyorhinis
  • M. arginini
  • M. orale 
  • M. salivarium
  • M. hominis
  • M. pulmonis
  • M. arthritidis
  • M. bovis
  • M. pneumoniae
  • M. pirum
  • M. capricolum 


Sensitivity

Type of Mycoplasma

Minimum Concentration Detected

M. FERMENTANS

240 CFU/mL

M. CAPRICOLOM

110 CFU/mL

PENETRANS

200 CFU/mL

HYORHINIS

210 CRU/mL