This antibody is a negative control for the E4NG antibody (product number 1061006). It is the mutated version of the E4NG antibody, with identical sequence, methods of production/purification and format to E4NG except two mutations in the paratope (W48M & S51A).

This antibody has neglectable or no affinity to citrulline and has no known cross-reactivity to other antigens. It may have minimal non- specific binding to CCP2, the cyclic citrullinated peptide(s) of the second generation of the anti-CCP test.

This antibody can be paired as a negative control with E4NG antibody (product number 1061006) for detecting citrullinated antigens. This negative control antibody is not sold separately.

Anti-citrullinated protein antibodies (ACPA) are autoantibodies linked to rheumatoid arthritis (RA). In RA research, collagen-induced arthritis (CIA) or CAIA mouse models are used to study ACPA's role in disease mechanisms like inflammation and joint damage.

Synonyms:
Citrullinated epitopes of COL2, Collagen alpha-1(II) chain, COL2A1, type II collagen, CII1; Citrullinated epitopes of ENO1, MPB1

The antibody clone E4NG (Catalogue number 1061006) binds to citrullinated collagen type II (COL2) epitopes. E4NG-mutant (Catalogue number 1061006-NC) is the control antibody that does not bind to citrulline. Binding to unmodified COL2 peptides is low by both E4NG and E4NG-mutant antibodies. A multiplex flow immunoassay determined binding. (Results published in He et al Nat Comm 2023)

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