ACPA (Negative Control), Monoclonal Antibody (Clone E4NG-Mutant), 100ug
This antibody is a negative control for the E4NG antibody (product number 1061006). It is the mutated version of the E4NG antibody, with identical sequence, methods of production/purification and format to...
Antibodies
1061006-NC
This antibody is a negative control for the E4NG antibody (product number 1061006). It is the mutated version of the E4NG antibody, with identical sequence, methods of production/purification and format to E4NG except two mutations in the paratope (W48M & S51A).
This antibody has neglectable or no affinity to citrulline and has no known cross-reactivity to other antigens. It may have minimal non- specific binding to CCP2, the cyclic citrullinated peptide(s) of the second generation of the anti-CCP test.
This antibody can be paired as a negative control with E4NG antibody (product number 1061006) for detecting citrullinated antigens. This negative control antibody is not sold separately.
Anti-citrullinated protein antibodies (ACPA) are autoantibodies linked to rheumatoid arthritis (RA). In RA research, collagen-induced arthritis (CIA) or CAIA mouse models are used to study ACPA's role in disease mechanisms like inflammation and joint damage.
Synonyms:
Citrullinated epitopes of COL2, Collagen alpha-1(II) chain, COL2A1, type II collagen, CII1; Citrullinated epitopes of ENO1, MPB1
The antibody clone E4NG (Catalogue number 1061006) binds to citrullinated collagen type II (COL2) epitopes. E4NG-mutant (Catalogue number 1061006-NC) is the control antibody that does not bind to citrulline. Binding to unmodified COL2 peptides is low by both E4NG and E4NG-mutant antibodies. A multiplex flow immunoassay determined binding. (Results published in He et al Nat Comm 2023)
References:
1. He, Y., Ge, C., Moreno-Giró, À. et al. A subset of antibodies targeting citrullinated proteins confers protection from rheumatoid arthritis. Nat Commun 14, 691 (2023).
Product Insert (PDF) - Informational use only. Please refer to insert included with product.
- Clone: E4NG-Mutant
- Host/Isotype: Mouse IgG2b, k
- Immunogen: Initially derived from a rheumatoid arthritis (RA) patient, selected by a single B cell isolated from an RA patient with high reactivity to cyclic citrullinated peptide 2 (CCP2). The antibody is recombinantly expressed in HEK cells as a chimeric antibody (human variable domain + mouse constant domain) except two mutations in the paratope (W48M & S51A).
- Species Cross-Reactivity: Mouse, Human
- Form: This antibody is soluble and supplied in PBS.
- Application: The E4NG-mutant antibody is recommended as a negative control for the E4NG antibody (product number 1061006) in assays detecting citrulline- specificity of anti-citrullinated protein antibodies (ACPA). It is packed with product number 1061006 only and not sold separately.
- Suggested Dilution: It is recommended that the user determine the optimal dilution for their application. The typical starting working dilutions are for IHC 1:100-1:500, ELISA 1:500-1:1000.
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