Collagen Type II, Monoclonal Antibody (Clone M2139), 100ug

This antibody (clone 2139) recognizes the conformational J1 epitope on the triple-helical structure of the native Collagen Type II molecule. The J1 epitope on mouse collagen consists of residues 551–564. It...

Antibodies

1061001

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For research use only. Not for use in diagnostic procedures.

This antibody (clone 2139) recognizes the conformational J1 epitope on the triple-helical structure of the native Collagen Type II molecule. The J1 epitope on mouse collagen consists of residues 551–564. It cross-reacts with mouse, rat, human, bovine, and chicken.

Collagen type II, encoded by the COL2A1 gene, is a crucial component of cartilage and plays a vital role in maintaining joint integrity. In mouse models of arthritis, such as collagen-induced arthritis, COL2A1 is studied for its role in cartilage degradation, a key feature of conditions like rheumatoid arthritis and osteoarthritis. Research on COL2A1 helps in understanding arthritis mechanisms and developing new treatments.

Synonyms:
Collagen alpha-1(II) chain, COL2A1, Collagen Type II, Collagen Type 2, CII.

 

Collagen Type II, Monoclonal Antibody (Clone M2139), 100ug

M2139 binds to the triple helical J1 epitope on collagen II. Binding to collagen II epitopes was determined in bead-based flow immunoassay as median fluorescence intensity (MFI). Triple helical (Gly-Pro- Hyp)x11 is a synthetic control peptide with 11 repeats of the Gly-Pro-Hyp sequence and mimics the collagen II backbone structure.

 

References:

1. Mo JA, Holmdahl R. The B cell response to autologous type II collagen: biased V gene repertoire with V gene sharing and epitope shift. J Immunol. 1996 Sep 15;157(6):2440-8.


2. Tong D, Lönnblom E, Yau ACY, Nandakumar KS, Liang B, Ge C, Viljanen J, Li L, Bãlan M, Klareskog L, Chagin AS, Gjertsson I, Kihlberg J, Zhao M, Holmdahl R. A Shared Epitope of Collagen Type XI and Type II Is Recognized by Pathogenic Antibodies in Mice and Humans with Arthritis. Front Immunol. 2018 Apr 12;9:451.

 

3. Uysal H, Bockermann R, Nandakumar KS, Sehnert B, Bajtner E, Engström A, Serre G, Burkhardt H, Thunnissen MM, Holmdahl R. Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis. J Exp Med. 2009 Feb 16;206(2):449- 62.

 

4. Li Y, Tong D, Liang P, Lönnblom E, Viljanen J, Xu B, Nandakumar KS, Holmdahl R. Cartilage-binding antibodies initiate joint inflammation and promote chronic erosive arthritis. Arthritis Res Ther. 2020 May 24;22(1):120.

 

5. Amirahmadi SF, Whittingham S, Crombie DE, Nandakumar KS, Holmdahl R, Mackay IR, van Damme MP, Rowley MJ. Arthritogenic anti- type II collagen antibodies are pathogenic for cartilage-derived chondrocytes independent of inflammatory cells. Arthritis Rheum. 2005 Jun; 52(6):1897-906.


Product Insert (PDF) - Informational use only. Please refer to insert included with product.

Data/Specifications:

  • Clone: M2139
  • Host/Isotype: Mouse IgG2b,k
  • Immunogen: Mouse collagen II J1 epitope (triple-helical position 551-564)
  • Species Cross-Reactivity: Mouse, Rat, Human, Bovine, Chicken
  • Form: This antibody is soluble and supplied in PBS.
  • Application: Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP), functional assays (in vivo and in vitro)
  • Suggested Dilution: It is recommended that the user determine the optimal dilution for their application. The typical starting working dilutions are for functional assays (cell culture) 1:20-1:50 and IHC 1:100.


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